Abstract
The specific activities of aldolase of cytosols isolated from the livers of 30–31-month-old rats were statistically significantly lower (P <.01) than those isolated from young animals. Although the present study provides consistent evidence for the presence of inactive aldolase in the cytosols isolated from old rats in both studies of immunoprecipitation (13–22% inactive enzyme molecules) and purification of the enzyme (15%), the age-associated changes were smaller than those previously reported for mouse. Since it is possible that there are intrinsic differences between rats and mice which may mask similar age differences, a number of variables which may influence or provide information regarding the synthesis of aldolase were also investigated in rats and mice. The data clearly indicated that in addition to age, rats and mice respond in a qualitative but not quantitative manner to dietary manipulation. For example, an increase in aldolase activity occurs in both rats and mice fed either a high fructose-no fat (HF-NF) or a low protein-high carbohydrate diet. However, the response was markedly greater in mice than in rats.
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Barrows, C.H., Kokkonen, G.C. The effect of age on aldolase in the livers of rats. AGE 3, 53–58 (1980). https://doi.org/10.1007/BF02431728
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DOI: https://doi.org/10.1007/BF02431728