Skip to main content
SpringerLink
Log in

A hybridization assay specific for ribosomal RNA fromEscherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Ribosomal RNA fromEscherichia coli forms specific hybrids with heterologous DNA fromVibrio metschnicovii. The conditions for such hybridization are described, in which the amount of mRNA fromE. coli binding to theVibrio DNA is about 3% of the amount which hybridizes to the same amount of DNA fromE. coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chargaff, E.: In: The nucleic acids, vol. 1, p. 307–371, Chargaff, E., Davidson, J. N. eds. New York: Academic Press 1965.

    Google Scholar 

  • Clark, D. J., Maaløe, O.: DNA replication and the division cycle inEscherichia coli. J. molec. Biol.23, 99–112 (1967).

    CAS  Google Scholar 

  • Colli, W., Oishi, M.: Ribosomal RNA genes in bacteria: Evidence for the nature of the physical linkage between 16S and 23S RNA genes inBacillus subtilis. Proc. nat. Acad. Sci. (Wash.)64, 642–649 (1969).

    Article  CAS  Google Scholar 

  • Forchhammer, J., Kjeldgaard, N. O.: Decay of messenger RNAin vivo in a mutant ofEscherichia coli 15. J. molec. Biol.24, 459–470 (1967).

    Article  CAS  Google Scholar 

  • Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol.12, 829–842 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Kohne, D. E.: Isolation and characterization of bacterial ribosome RNA cistrons. Biophys. J.8, 1104–1118 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Lavallé, R., DeHauwer, G.: Messenger RNA synthesis during amino acid starvation inEscherichia coli. J. molec. Biol.37, 269–288 (1968).

    Article  PubMed  Google Scholar 

  • Lindahl, L., Forchhammer, J.: Evidence for reduced breakdown of messenger RNA during blocked transcription or translation inEscherichia coli. J. molec. Biol.43, 593–606 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol.3, 208–218 (1961).

    CAS  Google Scholar 

  • Matzura, H., Molin, S., Maaløe, O.: Sequential biosynthesis of the β and β′ subunits of the DNA-dependent RNA polymerase fromEscherichia coli. J. molec. Biol.59, 17–25 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Miura, K.-I.: The nucleotide composition of ribonucleic acids of soluble and particle fractions in several species of bacteria. Biochim. biophys. Acta (Amst.)55, 62–70 (1962).

    Article  CAS  Google Scholar 

  • Nomura, M., Traub, P., Bechmann, H.: Hybrid 30S ribosomal particles reconstituted from components of different bacterial origins. Nature (Lond.)219, 793–799 (1968).

    Article  CAS  Google Scholar 

  • Schildkraut, C. L., Marmur, J., Doty, P.: The formation of hybrid DNA molecules and their use in studies of DNA homologies. J. molec. Biol.3, 593–617 (1961).

    Article  Google Scholar 

  • Sekiguchi, M., Iida, S.: Mutants ofEscherichia coli permeable to actinomycin. Proc. nat. Acad. Sci. (Wash.)58, 2315–2320 (1967).

    Article  CAS  Google Scholar 

  • Stamato, T. D., Pettijohn, D. E.: Regulation of ribosomal RNA synthesis in stringent bacteria. Nature (Lond.) New Biol.234, 99–102 (1971).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, University of Aarhus, DK-8000, Aarhus C, Denmark

    S. Pedersen &  N. O. Kjeldgaard

Authors
  1. S. Pedersen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. O. Kjeldgaard
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. Starlinger

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pedersen, S., Kjeldgaard, N.O. A hybridization assay specific for ribosomal RNA fromEscherichia coli . Molec. Gen. Genet. 118, 85–91 (1972). https://doi.org/10.1007/BF02428335

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02428335

Keywords

Search

Navigation