Summary
Two unlinked loci controlling the glucose-repressible alcohol dehydrogenase (ADH II) inSaccharomyces cerevisiae were investigated. One locus (AD R2) was characterized by electrophoreticallyslow andfast alleles and by inactiveadr2 mutant alleles. The ADH II pattern of heteroallelicslow × fast diploids indicates a tetrameric structure of the enzyme.AD R2 was considered as the structural gene, which codes for the ADH II subunits. Allelicadr2-f mutants could be classified by their response to theslow wild type allele (AD R2-S) in heter ozyg ous diploids. In most cases, only the slow band appeared. In threeadr2-f/AD R2-S crosses hybrid enzymes between inactive fast and active slow enzymes were formed. It was demonstrated, that allelic interactions at the protein level are not restricted to electrophoretical behaviour of hybrid enzymes. They also influence specific activities and substrate affinities.
The other locus investigated,AD R1, was characterized by ADH II negative mutants (adr1) and by allelic mutants which generate only very low activity (AD R1-L).ADR1 does not influence the electrophoretic properties of slow and fast ADH II proteins.adr1 mutants have an intact structural gene, which is not expressed. The gene has probably a regulatory function with respect to ADH II synthesis.
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Ciriacy, M. Genetics of alcohol dehydrogenase inSaccharomyces cerevisiac . Molec. Gen. Genet. 138, 157–164 (1975). https://doi.org/10.1007/BF02428119
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DOI: https://doi.org/10.1007/BF02428119