Abstract
The morphological study of the chromosomes ofNicotiana plumbaginifolia was carried out using two karyological techniques referred to as the squash method and the protoplast method. The latter involves isolation of protoplasts from unfixed root meristems and provides mitotic images in which the chromosomes, conveniently dispersed and disposed on the same plane, are significantly longer, and better structured, than those obtained by the squash method. A critical analysis of this technique is carried out. The results of this study led us to propose a karyotype forNicotiana plumbaginifolia and to characterize each chromosome by mere morphological examination. This characterization was further detailed by C-banding.
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References
Bourgin, J. P., Chupeau, Y. & Missionier, C., 1979. Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol. Plant. 45: 288–292.
Chien, Y. C., Kao, K. N. & Weter, L. R., 1982. Chromosomal and izozyme studies of Nicotiana tabacum-Glycine max hybrid cell lines. Theor. appl. Genet. 62: 301–304.
Gleba, Y. Y., Momot, V. P., Okolot, A. N., Cherep, N. N., Sharzhynskaya, M. V. & Kotov, V., 1983. Genetic processes in intergeneric cell hybrids Atropa + Nicotiana. 1. Genetic constitution of cells of different clonal origin grown in vitro. Theor. appl. Genet. 65: 269–276.
Goodspeed, T. H., 1954. The genus Nicotiana. Chronica bot. Waltham, Mass., U.S.A.
Harms, C. T., 1983. Somatic hybridization by plant protoplast fusion. In: Protoplasts 1983. Lecture proceedings. I. Potrykus & al. eds, Birkhäuser, Basel, pp. 69–84.
Kao, K. N., 1977. Chromosomal behaviour in somatic hybrids of soybean — Nicotiana glauca. Mol. gen. Genet. 150: 225–230.
Mouras, A., Salessess, G. & Lutz, A., 1978. Sur l'utilisation des protoplastes en cytologie: amélioration d'une méthode récente en vue de l'identification des chromosomes des genres Nicotiana et Prunus. Caryologia 31: 117–127.
Murashige, T. & Skoog, F., 1962. A revised medium for rapid growth and bio-assay with tobacco tissue culture. Physiol. Plant. 15: 473–497.
Negrutiu, I., 1981. Improved conditions for large-scale culture, mutagenesis, and selection of haploid protoplasts of Nicotiana plumbaginifolia Viviani. Z. Pflanzenphysiol. 104: 431–442.
Sidorov, P. & Maliga, P., 1982. Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures. Mol. gen. Genet. 186: 328–332.
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Mouras, A., Wildenstein, C. & Salesses, G. Analysis of karyotype and C-banding pattern ofNicotiana plumbaginifolia using two techniques. Genetica 68, 197–202 (1986). https://doi.org/10.1007/BF02424443
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DOI: https://doi.org/10.1007/BF02424443