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Intracellular coding sites of polypeptides associated with photosynthetic oxygen evolution of photosystem II

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Summary

Three hydrophilic polypeptides of approximately 34, 23, and 16 kd located on the inner thylakoid surface are associated with the water-splitting activity of photosystem II. Stable transcripts for the three proteins were found only in cytosolic (polyadenylated) RNA, suggesting that they are encoded in nuclear genes. The immunologically reacting products synthesized in a rabbit reticulocyte cell-free translation system are larger in size than the authentic mature proteins by about 6–10 kd. These larger precursors are imported post-translationally into isolated, intact chloroplasts, and are processed to their mature forms during or after translocation. The imported proteins can be extracted from thylakoids by procedures used to isolate the three native proteins of the water-splitting complex, suggesting that they have assembled properly into their final destination, the inner thylakoid surface.

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Authors and Affiliations

  1. Botanisches Institut der Universität, Universitätsstraße 1, 4 Düsseldorf 1, FRG

    Peter Westhoff

  2. Department of Biochemistry, University of Lund, Sweden

    Christer Jansson & Christer Larsson

  3. Biochemie der Pflanze, Ruhr-Universität Bochum, FRG

    Ludger Klein-Hitpaß & Richard Berzborn

  4. Department of Biochemistry, Louisiana State University, Baton Rouge, La., USA

    Sue G. Bartlett

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  1. Peter Westhoff
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  2. Christer Jansson
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  4. Richard Berzborn
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  5. Christer Larsson
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  6. Sue G. Bartlett
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Westhoff, P., Jansson, C., Klein-Hitpaß, L. et al. Intracellular coding sites of polypeptides associated with photosynthetic oxygen evolution of photosystem II. Plant Mol Biol 4, 137–146 (1985). https://doi.org/10.1007/BF02418761

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  • DOI: https://doi.org/10.1007/BF02418761

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