Abstract
Two different types of contacts (or interfaces) exist between the plant host and the fungus during the vesicular-arbuscular mycorrhizal symbiosis, depending on whether the fungus is intercellular or intracellular. In the first case, the walls of the partners are in contact, while in the second case the fungal wall is separated from the host cytoplasm by the invaginated host plasmamembrane and by an interfacial material. In order to verify the origin of the interfacial material, affinity techniques which allow identification in situ of cell-wall components, were used. Cellobiohydrolase (CBH I) that binds to cellulose and a monoclonal antibody (JIM 5) that reacts with pectic components were tested on roots ofAllium porrum L. (leek) colonized byGlomus versiforme (Karst.) Berch. Both probes gave a labelling specific for the host cell wall, but each probe labelled over specific and distinct areas. The CBH I-colloidal gold complex heavily labelled the thick epidermal cell walls, whereas JIM 5 only labelled this area weakly. Labelling of the hypodermis was mostly on intercellular material after treatment with JIM 5 and only on the wall when CBH I was used. Suberin bands found on the radial walls were never labelled. Cortical cells were mostly labelled on the middle lamella with JIM 5 and on the wall with CBH I. Gold granules from the two probes were found in interfacial material both near the point where the fungus enters the cell and around the thin hyphae penetrating deep into the cell. The ultrastructural observations demonstrate that cellulose and pectic components have different but complementary distributions in the walls of root cells involved in the mycorrhizal symbiosis. These components show a similar distribution in the interfacial material laid down around the vesicular-arbuscular mycorrhizal fungus indicating that the interfacial material is of host origin.
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Abbreviations
- CBH I:
-
cellobiohydrolase
- CMC:
-
oarboxymethylcellulose
- JIM 5:
-
anti-pectin monoclonal antibody
- PATAg:
-
periodic acid-thiocarbohydrazide-silver proteinate
- VAM:
-
vesicular-arbuscular mycorrhiza
References
Bartnicki-Garcia, S. (1970) Cell wall composition and other biochemical markers in fungal phylogeny. In: Phytochemical phylogeny. pp. 81–103, Harborne, J.B., ed., Academic Press, London
Benhamou, N. (1988) Ultrastructural localization of carbohydrates in the cell walls of two pathogenic fungi: a comparative study. Mycologia80, 324–337
Benhamou, N., Gilboa-Garber, N., Trudel, J., Asselin, A. (1988) A new lectin-gold complex for ultrastructural localization of galacturonic acids. J. Histochem. Cytochem.3, 1403–1411
Berg, R.H., Erdos, G.W., Gritzali, M., Brown, R.D. Jr. (1988) Enzyme-gold affinity labelling of cellulose. J. Electron Microsc. Tech.8, 371–379
Bonfante-Fasolo, P. (1984) Anatomy and morphology of VA mycorrhizae. In: VA mycorrhizas, pp. 5–33, Powell, C.L., Bagyaraj, D.J., eds. CRC Press, Boca Raton, Fla., USA
Bonfante-Fasolo, P., Vian, B. (1989) Cell wall texture in symbiotic toots ofAllium porrum L. Ann. Sci. Nat. Bot.10, 97–109
Bonfante-Fasolo, P., Faccio, A., Perotto, S., Schubert, A. (1990) Correlation between chitin distribution and cell wall morphology in the mycorrhizal fungusGlomus versiforme. Mycol. Research94, 157–165
Bracker, C.E., Littlefield, L.J. (1973) Structural concepts of hostpathogen intefaces. In: Fungal pathogenicity and the plant's response, pp. 159–317, Byrde, R.J.W., Cutting, C.V., eds. Academic Press, London
Bradley, D.J., Wood, E.A., Larkins, A.P., Galfrè, G., Butcher, G.W., Brewin, N.J. (1988) Isolation of monoclonal antibodies reacting with peribacteroid membranes and other components of pea root nodules containingRhizobium leguminosarum. Planta173, 149–160
Cervone, F., Castoria, R., Spanu, P., Bonfante-Fasolo, P. (1988) Pectinolytic activity in some ericoid mycorrhizal fungi. Trans. Br. Mycol. Soc.91, 537–539
Chanzy, H., Henrissat, B., Voung, R. (1984) Colloidal gold labelling of 1,4, β-d-glucan cellobiohydrolase adsorbed on cellulose substrate. FEBS Lett.172, 193–197
Dexheimer, J., Gianinazzi, S., Gianinazzi-Pearson, V. (1979) Ultrastructural cytochemistry of the host-fungus interface in the endomycorrhizal associationGlomus mosseae/Allium cepa. Z. Pflanzenphysiol.86, 189–201
Dumas, E., Gianinazzi-Pearson, V., Gianinazzi, S. (1989) Production of new soluble proteins during VA endomycorrhiza formation. In: Agricultural ecosystems and environments, Mejstrik, V., ed. Elsevier, Amsterdam (in press)
Harley, J.L., Smith, S.E. (1983) Mycorrhizal symbiosis. Academic Press, London
Hewitt, E.J. (1966) Sand and water culture methods used in the study of plant nutrition, pp. 187–237, Commonwealth Agricultural Bureaux Farnhan Royal, Bucks., UK
Hilbert, J.L., Martin, F. (1988) Regulation of gene expression in ectomycorrhizas. I. Protein changes and the presence of ectomycorrhiza specific polypeptides in thePisolithus-Eucalyptus symbiosis. New Phytol.110, 339–346
Keon, J.P.R., Byrde, R.J.W., Cooper, R.M. (1987) Some aspects of fungal enzymes that degrade plant cell walls. In: Fungal infection of plants, pp. 133–157, Pegg, G.F., Ayres, P.G., eds. Cambridge University Press, Cambridge, UK
Jarvis, M.C., Forsyth, W., Duncan, H.J. (1988) A survey of the pectic content of nonlignified monocot cell walls. Plant Physiol.88, 309–314
Jeffries, P. (1987) Pathways for the exchange of materials in mycoparasitic and plant-fungal interactions. In: Fungal infection of plants, pp. 60–78, Pegg, G.F., Ayres, P.G. eds. Cambridge University Press, Cambridge, UK
McNeil, M., Darvill, G.A., Fry, C.S., Albersheim, P. (1984) Structure and function of the primary cell wall of plants. Annu. Rev. Biochem.53, 625–663
Moore, P.J., Staehelin, L.A. (1988) Immunogold localization of the cell-wall-matrix polysaccharides rhamnogalacturonan I and xyloglucan during cell expansion and cytokinesis inTrifolium pratense L.; implication for secretory pathways. Planta174, 433–445
Northcote, D.H., Davey, R., Lay, J. (1989) Use of antisera to localize callose, xylan and arabinogalactan in the cell-plate, primary and secondary walls of plant cells. Planta178, 353–366
Redgwell, R.J., Selvendran, R.R. (1986) Structural features of cell-wall polysaccharides of onionAllium cepa. Carbohydr. Res.157, 183–199
Roland, J.C., 1978. General preparations and staining of thin sections. In: Electron microscopy and cytochemistry of plant cells, pp. 1–62, Hall, J.L., ed. Elsevier, Amsterdam
Scannerini, S., Bonfante-Fasolo, P. (1979) Ultrastructural cytochemical demonstration of polysaccharides and proteins within host-arbuscule interfacial matrix in an endomycorrhiza. New Phytol.83, 739–744
Scannerini, S., Bonfante-Fasolo, P. (1983) Comparative ultrastructural analysis of mycorrhizal associations. Can. J. Bot.61, 917–943
Schulein, M. (1988) Cellulase ofTrichoderma reesei. Methods Enzymol.160, 234–242
Spanu, P., Boller, T., Ludwig, A., Wiemken, A., Faccio, A., Bonfante-Fasolo, P. (1989) Chitinase in roots of mycorrhizalAllium porrum: regulation and localization. Planta177, 447–455
Toth, R., Miller, R.M. (1984) Dynamics of arbuscule development and degeneration in aZea mays mycorrhiza. Am. J. Bot.71, 449–460
VandenBosch, K.A., Bradley, D.J., Knox, J.P., Perotto, S., Butcher, G.W., Brewin, N.J. (1989) Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots. EMBO J.8, 335–342
Vian, B. (1986) Ultrastructural localization of carbohydrates. Recent developments in cytochemistry and affinity methods. In: Biology and molecular biology of plant-pathogen interactions, pp. 49–57, Bailey, J.A., ed. Springer, Berlin Heidelberg New York
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Bonfante-Fasolo, P., Vian, B., Perotto, S. et al. Cellulose and pectin localization in roots of mycorrhizalAllium porrum: labelling continuity between host cell wall and interfacial material. Planta 180, 537–547 (1990). https://doi.org/10.1007/BF02411452
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DOI: https://doi.org/10.1007/BF02411452