Abstract
Intact leaves of kiwifruit (Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson) from plants grown in a range of controlled temperatures from 15/10 to 30/25°C were exposed to a photon flux density (PFD) of 1500 μmol·m−2·s−1 at leaf temperatures between 10 and 25°C. Photoinhibition and recovery were followed at the same temperatures and at a PFD of 20 μmol·m−2·s−1, by measuring chlorophyll fluorescence at 77 K and 692 nm, by measuring the photon yield of photosynthetic O2 evolution and light-saturated net photosynthetic CO2 uptake. The growth of plants at low temperatures resulted in chronic photoinhibition as evident from reduced fluorescence and photon yields. However, low-temperature-grown plants apparently had a higher capacity to dissipate excess excitation energy than leaves from plants grown at high temperatures. Induced photoinhibition, from exposure to a PFD above that during growth, was less severe in low-temperature-grown plants, particularly at high exposure temperatures. Net changes in the instantaneous fluorescence,F 0, indicated that little or no photoinhibition occurred when low-temperature-grown plants were exposed to high-light at high temperatures. In contrast, high-temperature-grown plants were highly susceptible to photoinhibitory damage at all exposure temperatures. These data indicate acclimation in photosynthesis and changes in the capacity to dissipate excess excitation energy occurred in kiwifruit leaves with changes in growth temperature. Both processes contributed to changes in susceptibility to photoinhibition at the different growth temperatures. However, growth temperature also affected the capacity for recovery, with leaves from plants grown at low temperatures having moderate rates of recovery at low temperatures compared with leaves from plants grown at high temperatures which had negligible recovery. This also contributed to the reduced susceptibility to photoinhibition in low-temperature-grown plants. However, extreme photoinhibition resulted in severe reductions in the efficiency and capacity for photosynthesis.
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Abbreviations
- F 0 ,F m ,F v :
-
instantaneous, maximum, variable fluorescence
- F v/F m :
-
fluorescence ratio
- F i :
-
F v att=0
- F c :
-
F v of control leaves
- F ∞ :
-
F v att=∞
- K D :
-
rate constant for non-radiative energy dissipation
- K P :
-
rate constant for photochemistry
- k(F p):
-
first-order rate constant for photoinhibition
- k(F r):
-
first-order rate constant for recovery
- PFD:
-
photon flux density
- P s :
-
light-saturated net photosynthesis
- ϕ i :
-
photon yield of O2 evolution (incident light)
References
Anderson, J.M., Andersson, B. (1988) The dynamic photosynthetic membrane and regulation of solar energy conversion. Trends Biochem. Sci.13, 351–355
Björkman, O. (1987) Low-temperature chlorophyll fluorescence in leaves and its relationship to photon yield of photosynthesis in photoinhibition. In: Topics in photosynthesis, vol. 9: Photoinhibition, pp. 123–144, Kyle, D.J., Osmond, C.B., Arntzen, C.J. eds. Elsevier, Amsterdam
Björkman, O., Demmig, B. (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta170, 489–504
Cleland, R.E., Melis, A., Neale, P.J. (1986) Mechanism of photoinhibition: photochemical reaction centre inactivation in system II of chloroplasts. Photosynth. Res.9, 79–88
Demmig, B., Björkman, O. (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of O2 evolution in leaves of higher plants. Planta171, 171–184
Demmig, B., Winter, K., Krüger, A., Czygan, F.C. (1987) Photoinhibition and zeaxanthin formation in intact leaves. A possible role of the xanthophyll cycle in the dissipation of excess light energy. Plant Physiol.84, 218–224
Demmig, B., Winter, K., Krüger, A., Czygan, F.-C. (1988) Zeaxanthin and the heat dissipation of excess light energy inNerium oleander exposed to a combination of high light and water stress. Plant Physiol87, 17–24
Greer, D.H. (1988) Effect of temperature on photoinhibition and recovery inActinidia deliciosa. Aust. J. Plant Physiol.15, 195–205
Greer, D.H., Berry, J.A., Björkman, O. (1986) Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta168, 253–260
Greer, D.H., Hardacre, A.K. (1989) Photoinhibition of photosynthesis and its recovery in two maize hybrids varying in low temperature tolerance. Aust. J. Plant Physiol.16, 189–198
Greer, D.H., Laing, W.A. (1988a) Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: recovery and its dependence on temperature. Planta174, 159–165
Greer, D.H., Laing, W.A. (1988b) Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: effect of light during growth on photoinhibition and recovery. Planta175, 355–363
Greer, D.H., Laing, W.A., Kipnis, T. (1988) Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: effect of temperature. Planta174, 152–158
Knapp, A.K., Vogelmann, T.C., McClean, T.M., Smith, W.K. (1988) Light and chlorophyll gradients withinCucurbita cotyledons. Plant Cell Environ11, 257–263
Krause, G.H., Somersalo, S. (1988) Fluorescence as a tool in photosynthesis research; application in studies of photoinhibition, cold acclimation and freezing stress. Philos. Trans. R. Soc. Lond. [Biol]323, 281–294
Laing, W.A. (1985) Temperature and light response curves for photosynthesis in kiwifruit (Actinidia chinensis) cv. Hayward. N.Z. J. Agric. Res.28, 117–124
Moll, B.A., Steinback, K.E. (1986) Chilling sensitivity inOryza sativa: The role of protein phosphorylation in protection against photoinhibition. Plant Physiol.80, 420–423
Morgan, D.C., Warrington, I.J., Halligan, E.A. (1985) Effect of temperature and photosynthetic photon flux density on vegetative growth of kiwifruit (Actinidia chinensis) N.Z. J. Agric. Res.28, 109–116
Ohad, I., Kyle, D.J., Arntzen, C.J. (1984) Membrane protein damage and repair: removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes. J. Cell Biol.99, 481–485
Öquist, G., Greer, D.H., Ogren, E. (1987) Light stress at low temperature. In: Topics in photosynthesis, vol. 9: Photoinhibition, pp. 67–87, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam
Powles, S.B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol.35, 15–44
Powles, S.B., Björkman, O. (1982) Photoinhibition of photosynthesis: Effect on chlorophyll fluorescence at 77 K in intact leaves and in chloroplast membranes ofNerium oleander. Planta156, 97–107.
Powles, S.B., Critchley, C. (1980) Effect of light intensity during growth on photoinhibition of attached intact bean leaflets. Plant Physiol.65, 1181–1187
Powles, S.B., Thorne, S.W. (1981) Effect of high-light treatments in inducing photoinhibition of photosynthesis in intact leaves of low-light grownPhaseolus vulgaris andLastreopsis microsora. Planta152, 471–477
Seemann, J.R., Sharkey, T.D., Wang, J., Osmond, C.B. (1987) Environmental effects on photosynthesis, nitrogen-use efficiency, and metabolite pools in leaves of sun and shade plants. Plant Physiol.84, 796–802
Smillie, R.M., Hetherington, S.E., He, J., Nott, R. (1988) Photoinhibition at chilling temperatures. Aust. J. Plant Physiol.15, 207–222
Thompson, L.K., Brudvig, G.W. (1988) Cytochromeb-559 may function to protect photosystem II from photoinhibition. Biochemistry27, 6653–6658
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Greer, D.H., Laing, W.A. Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: effect of growth temperature on photoinhibition and recovery. Planta 180, 32–39 (1989). https://doi.org/10.1007/BF02411407
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DOI: https://doi.org/10.1007/BF02411407