Skip to main content
SpringerLink
Log in

Influence of a diphosphonate on the cellular aspect of young bone tissue

  • Laboratory Investigation
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

Stimulated by the rather sparse information in the literature on cellular changes induced by EHDP, we carried out electron microscopic investigations on young bone tissue and on de novo bone formation. Cellular changes could be observed during continuous administration of EHDP. The osteoblasts demonstrated temporary storing of crystalloid structures in the mitochondria, and atypical osteocytes showed persistent changes indicative of hyperactivity. The osteoclasts exhibited varying ultrastructural features with respect to the number and appearance of nuclei, Golgi, RER, and lysosomes. These changes under the influence of EHDP could be an indication of altered activity of the osteoclast. The possible interference of EHDP with bone cell metabolism is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fleisch, H., Russell, R.G.G., Bisaz, S., Casey, P.A., Mühlbauer, R.: The influence of pyrophosphate analogues (diphosphonates) on the precipitation and dissolution of calcium phosphate in vitro and in vivo, Calcif. Tissue Res. [Suppl.]2:10–10A, 1968.

    Article  Google Scholar 

  2. Russell, R.G.G., Mühlbauer, R.C., Bisaz, S., Williams, D.A., Fleisch, H.: The influence of pyrophosphate, condensed phosphates, phosphonates and other phosphate compounds on the dissolution of hydroxyapatite in vitro and on bone resorption induced by parathyroid hormone in tissue culture and in thyroparathyroidectomised rats, Calcif. Tissue Res.6:183–196, 1970.

    Article  CAS  PubMed  Google Scholar 

  3. Reynolds, J.J., Minkin, C., Morgan, D.B., Spycher, D., Fleisch, H.: The effect of two diphosphonates on the resorption of mouse calvaria in vitro, Calcif. Tissue Res.10:302–313, 1972.

    Article  CAS  PubMed  Google Scholar 

  4. King, W.R., Francis, M.D., Michaël, W.R.: Effect of disodium ethane-1-hydroxy-1, 1-diphosphonate on bone formation, Clin. Orthop.78:251–270, 1971.

    CAS  PubMed  Google Scholar 

  5. Russell, R.G.G., Kislig, A.M., Casey, P.A., Fleisch, H., Thornton, J., Schenk, R., Williams, D.A.: Effect of diphosphonates and calcitonin on the chemistry and quantitative histology of rat bone, Calcif. Tissue Res.11:179–195, 1973.

    CAS  PubMed  Google Scholar 

  6. Schenk, R., Merz, W.A., Mühlbauer, R., Russell, R.G.G., Fleisch, H.: Effect of ethane-1-hydroxy-1, 1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl2MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats, Calcif. Tissue Res.11:196–214, 1973.

    CAS  PubMed  Google Scholar 

  7. Francis, M.D.: The inhibition of calcium hydroxyapatite crystal growth by polyphosphonates and polyphosphates, Calcif. Tissue Res.3:151–162, 1969.

    Article  CAS  PubMed  Google Scholar 

  8. Wöltgens, J.H.M., Bonting, S.L., Bijvoet, O.L.M.: Influence of sodium ethane-1-hydroxy-1, 1-diphosphonate and Mg2+ on the inorganic pyrophosphatase in calcifying hamster molars, Isr. J. Med. Sci.7:406, 1971.

    PubMed  Google Scholar 

  9. Wöltgens, J.H.M., Bonting, S.L., Bijvoet, O.L.M.: Inorganic pyrophosphatases in mineralizing hamster molars. III. Influence of diphosphonates, Calcif. Tissue Res.13:151–157, 1973.

    Article  PubMed  Google Scholar 

  10. Guilland, D.F., Sallis, J.D., Fleisch, H.: The effect of two diphosphonates on the handling of calcium by rat kidney mitochondria in vitro, Calcif. Tissue Res.15:303–314, 1974.

    Article  CAS  PubMed  Google Scholar 

  11. Larsson, A., Larsson, S.-E.: Light microscopic and ultrastructural observations on the short-term effects of ethylene-1-hydroxy-1, 1-diphosphonate (EHDP) on rat tibia epiphysis, Acta Path. Microbiol. Scand. [A]84:17–27, 1976.

    CAS  Google Scholar 

  12. Larsson, A., Larsson, S.-E.: The effects of ethylene-1-hydroxy-1, 1-diphosphonate on cellular transformation and organic matrix of the epiphyseal growth plate of the rat. A light microscopic and ultrastructural study, Acta Pathol. Microbiol. Scand. [A]86:211–223, 1978.

    CAS  Google Scholar 

  13. Rosenblum, I.Y., McCuskey, R.S., McNeal, N.C., Kirckaert, G.A., Flora, L., Metzger, C.A.: The effects of EHDP on regenerating trabecular bone using in vivo microscopic, light and electron microscopic and electron microprobe techniques, Calcif. Tissue Res.20:91–104, 1976.

    CAS  PubMed  Google Scholar 

  14. Bisaz, S., Schenk, R., Kunin, A., Mühlbauer, R., Fleisch, H.: Effect of ethane-1-hydroxy-1, 1-diphosphonate and vitamin D deficiency on lactate production of epiphyseal and articular chick cartilage in vitro. Proc. 9th Europ. Symp. on Calcif. Tissue. Baden Vienna, pp. 305–309, 1972.

  15. Rowe, D.J., Hausmann, E.: The alteration of osteoclast morphology by diphosphonates in bone organ culture, Calcif. Tissue Res.20:53–60, 1976.

    CAS  PubMed  Google Scholar 

  16. Urist, M.R., Silverman, B.F., Büring, K., Dubuc, F.L., Rosenberg, J.M.: The bone induction principle, Clin. Orthop.53:243–283, 1967.

    CAS  PubMed  Google Scholar 

  17. Urist, M.R., Dowell, T.A., Hay, Ph. H., Strates, B.S.: Inductive substrates for bone formation, Clin. Orthop.59:59–96, 1968.

    CAS  PubMed  Google Scholar 

  18. Urist, M.R., Hay, Ph. H., Dubuc, F., Büring, K.: Osteogenetic competence, Clin. Orthop.64:194–220, 1969.

    CAS  PubMed  Google Scholar 

  19. Plasmans, C.M.T., Kuijpers, W., Slooff, T.J.J.H.: The effect of ethane-1-hydroxy-1, 1-diphosphonic acid (EHDP) on matrix induced ectopic bone formation, Clin. Orthop.132:233-244, 1978.

    CAS  PubMed  Google Scholar 

  20. Putte, van de, K. A., Urist, M.R.: Osteogenesis in the interior of intramuscular implants of decalcified bone matrix, Clin. Orthop.43:257–270, 1965.

    PubMed  Google Scholar 

  21. Bernard, T., Afzelius, B.: The matrix granules of mitochondria: a review, Sub-Cell. Biochem.1:375–389, 1972.

    Google Scholar 

  22. Nunez, E.A., Horwith, M., Krook, L., Whalen, J.P.: An electron microscopic investigation of human familial bone dysplasia, Am. J. Pathol.94:1–10, 1979.

    CAS  PubMed  Google Scholar 

  23. Marinozzi, V., Derenzini, M., Nardi, F., Gallo, P.: Mitochondrial inclusions in human cancer of the gastrointestinal tract, Cancer Res.37:1556–1563, 1977.

    CAS  PubMed  Google Scholar 

  24. Weisbrode, S.E., Capen, C.C., Pendley, C.B.: Effect of dichloromethylene diphosphonate on morphology, enzyme activity, and ash content of bones of thyroparathyroidectomised rats, Calcif. Tissue Res.25:119–126, 1978.

    Article  CAS  PubMed  Google Scholar 

  25. Parfitt, A.M.: II. PTH and bone cells: bone turnover and plasma calcium regulation. Metabolism25:909–955, 1976.

    Article  CAS  PubMed  Google Scholar 

  26. Miller, S.C., Jee, W.S.S.: The comparative effects of dichloromethylene diphosphonate (Cl2MDP) and ethane-1-hydroxy-1, 1-diphosphonate (EHDP) on growth and modeling of the rat tibia, Calcif. Tissue Res.23:207–214, 1977.

    Article  CAS  PubMed  Google Scholar 

  27. Gasser, A.B., Morgan, D.B., Fleisch, H., Richelle, L.J.: The influence of two diphosphonates on calcium metabolism in the rat, Clin. Sci.43:31–45, 1972.

    CAS  PubMed  Google Scholar 

  28. Parfitt, A.M.: The actions of parathyroid hormone on bone: relation to bone remodelling and turnover, calcium homeostasis and metabolic bone disease. I. Mechanisms of calcium transfer between blood and their cellular basis: morphological and kinetic approaches to bone turnover, Metabolism25:809–844, 1976.

    Article  CAS  PubMed  Google Scholar 

  29. Strates, B.S., Firschein, H.E., Urist, M.R.: Alkaline phosphatases and failure of calcification under the influence of a diphosphonate, Biochim. Biophys. Acta244:121–142, 1971.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Orthopaedics, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands

    C. M. T. Plasmans

  2. Laboratory of Cytology and Histology, The Netherlands

    P. H. K. Jap & Mietske Wijers

  3. Department of Otorhinolaryngology, Catholic University Nijmegen, The Netherlands

    W. Kuijpers

  4. Department of Orthopaedics, Catholic University Nijmegen, The Netherlands

    T. J. J. H. Slooff

Authors
  1. C. M. T. Plasmans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. H. K. Jap
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Kuijpers
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. J. J. H. Slooff
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mietske Wijers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Plasmans, C.M.T., Jap, P.H.K., Kuijpers, W. et al. Influence of a diphosphonate on the cellular aspect of young bone tissue. Calcif Tissue Int 32, 247–256 (1980). https://doi.org/10.1007/BF02408548

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02408548

Key words

Search

Navigation