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An osteo-inductive bone matrix extract stimulates thein vitro conversion of mesenchyme into chondrocytes

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Summary

Urea and guanidine extracts of demineralized beef and rabbit bone matrix were assayed bothin vivo andin vitro. One month following intramuscular implantation into mouse thighs, these extracts induced ectopic cartilage and bone. Seven days following continuousin vitro exposure to the same extracts, stage 24 chick limb bud mesenchymal cells in cultures had differentiated into greater numbers of chondrocytes than controls. These results suggest the feasibility of using limb bud mesenchymal cell cultures as anin vitro assay for bone matrix derived, extractable bioactive factors which effect the conversion of mesenchymal cells into chondrocytes as a requisite step inin vivo osteogenesis.

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Authors and Affiliations

  1. Dept. of Biology, Case Western Reserve University, 44106, Cleveland, Ohio

    Glenn T. Syftestad &  Arnold I. Caplan

  2. Bone Research Laboratory, Nuffield Orthopaedic Centre, Oxford University, Oxford, England

    James T. Triffitt

  3. Bone Research Laboratory, Dept. of Orthopaedic Surgery, UCLA, 90024, Los Angeles, California

    Marshall R. Urist

Authors
  1. Glenn T. Syftestad
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  2. James T. Triffitt
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  3. Marshall R. Urist
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  4. Arnold I. Caplan
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Syftestad, G.T., Triffitt, J.T., Urist, M.R. et al. An osteo-inductive bone matrix extract stimulates thein vitro conversion of mesenchyme into chondrocytes. Calcif Tissue Int 36, 625–627 (1984). https://doi.org/10.1007/BF02405378

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  • DOI: https://doi.org/10.1007/BF02405378

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