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Xenografts of articular chondrocytes in the nude mouse

  • Laboratory Investigations
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Summary

Subcutaneous transplantation of articular chondrocytes isolated enzymatically from immature rabbits and dogs into athymic (nu/nu) mice resulted in the formation of hyaline cartilaginous nodules. Graft rejection was seen when the cells were injected into heterozygous (nu/+) mice. Radiosulfate-labeled proteoglycan extracted from the xenografts had a high buoyant density and was digested by chondroitinase ABC. A monomeric preparation of proteoglycan (A1-D1) contained a small quantity of aggregate as assessed by gel chromatography and gel electrophoresis. Almost no incorporation of3H-thymidine was found by autoradiography. The matrix did not become calcified over the course of 42 days. The nude mouse system lends itself to testing a variety of problems in the biology of cartilage. These include the redifferentiation of chondrocytes following dedifferentiation in vitro. Species differences were found in this regard. The nodules formed by rabbit articular chondrocytes, grown in monolayer culture for up to 14 days, had a hyaline chondroid character. Dog chondrocytes that had “dedifferentiated,” during 14 days of culture prior to transplantation, formed a graft that had a sparse fibrous rather than hyaline matrix.

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References

  1. Green WT Jr (1977) Articular cartilage repair. Behavior of rabbit chondrocytes during tissue culture and subsequent allografting. Clin Orthop 124:237–250.

    CAS  PubMed  Google Scholar 

  2. Elves MW (1978) The immunobiology of joints. In: Sokoloff L (ed) The joints and synovial fluid, vol 1. Academic Press, New York, pp 331–406

    Google Scholar 

  3. Bentley G, Smith AU, Mukerjhee R (1978) Isolated epiphyseal chondrocyte allografts into joint surfaces. An experimental study in rabbits. Ann Rheum Dis 37:449–458

    Article  CAS  PubMed  Google Scholar 

  4. Thyberg J, Moskalewski S (1979) Bone formation in cartilage produced by transplanted epiphyseal chondrocytes. Cell Tissue Res 204:77–94

    Article  CAS  PubMed  Google Scholar 

  5. Loeb L (1926) Autotransplantation and homoiotransplantation of cartilage in the guinea-pig. Am J Pathol 2:111–122

    Google Scholar 

  6. Heyner S (1969) The significance of intercellular matrix in the survival of cartilage allografts. Transplantation 8:666–677

    CAS  PubMed  Google Scholar 

  7. London WT, Fucillo DA, Anderson B, Sever JL (1970) Concentration of rubella virus in chondrocytes of congenitally infected rabbits. Nature 226:172–173

    Article  CAS  PubMed  Google Scholar 

  8. Malseed ZM, Heyner S (1976) Antigenic profile of the rat chondrocytes. Arthritis Rheum 19:223–231

    CAS  PubMed  Google Scholar 

  9. Gertzbein SD, Tait JH, Devlin S, Rogargues S (1977) The antigenicity of chondrocytes. Immunology 33:141–145

    CAS  PubMed  Google Scholar 

  10. Urist MR, Adam T (1968) Cartilage or bone induction by articular cartilage. Observations with radioisotope labelling techniques. J Bone Joint Surg 50B:198–215

    Google Scholar 

  11. Kaminski J, Kaminski G, Moskalewski S (1980) Species differences in the ability of isolated eipiphyseal chondrocytes to hypertrophy after transplantation into the wall of the syrian hamster cheek pouch. Folia Biol (Krakow) 28:27–38

    CAS  Google Scholar 

  12. Gullino PM, Ediger RD, Giovanella B, Merchant B, Outzen HC, Reed ND, Wortis HH (1976) Guide for the care and use of the nude (thymus-deficient) mouse in biomedical research. ILAR News 19 (2):M1-M20

    Google Scholar 

  13. Sokoloff L, Malemud CJ, Green WT Jr (1970) Sulfate incorporation by articular chondrocytes in monolayer culture. Arthritis Rheum 13:118–124

    CAS  PubMed  Google Scholar 

  14. Green WT Jr (1971) Behavior of articular chondrocytes in cell culture. Clin Orthop 75:248–260

    PubMed  Google Scholar 

  15. Lillie RD (1965) Histopathologic technique and practical histochemistry, 3rd edn. McGraw-Hill, New York

    Google Scholar 

  16. Krystal G, Morris GM, Lipman JM, Sokoloff L (1983) DNA repair by articular chondrocytes. I. Unscheduled DNA synthesis following ultraviolet irradiation in monolayer culture. Mech Ageing Dev 21:83–96.

    Article  CAS  PubMed  Google Scholar 

  17. Prins APA, Lipman JM, McDevitt CA, Sokoloff L (1982) The effect of purified growth factors on rabbit articular chondrocytes in monolayer culture. 2. Sulfated proteoglycans. Arthritis Rheum 16:1228–1238

    Google Scholar 

  18. McDevitt CA (1981) The proteoglycans of hyaline cartilage and the intervertebral disc in ageing and osteoarthritis. In: Glynn LE (ed) Handbook of inflammation, vol 3: Tissue repair and remodeling. Elsevier-Holland, Amsterdam, pp 111–143

    Google Scholar 

  19. McDevitt CA, Muir H (1971) Gel-electrophoresis of proteoglycans and glycosaminoglycans on large pore composite polyacrylamide—agarose gels. Anal Biochem 44:612–622

    Article  CAS  PubMed  Google Scholar 

  20. Oike YO, Kimata K, Shinomura T, Nakazawa K, Suzuki S (1980) Structural analysis of chick embryo cartilage proteoglycan by selective degradation with chondroitin lyases (chondroitinases) and endo-β-D-galactosidase (keratanase). Biochem J 191:193–207

    CAS  PubMed  Google Scholar 

  21. Dahlberg A, Dingman CW, Peacock AC (1969) Electrophoretic characterization of bacterial polyribosomes in agarose—acrylamide gels. J Mol Biol 41:139–147

    Article  CAS  PubMed  Google Scholar 

  22. Moskalewski S, Kawiak J (1965) Cartilage formation after homotransplantation of isolated chondrocytes. Transplantation 3:737–747

    CAS  PubMed  Google Scholar 

  23. Chesterman PV, Smith AU (1968) Homotransplantation of articular cartilage and isolated chondrocytes. J Bone Joint Surg 50B:184–215

    Google Scholar 

  24. Moskalewski S (1981) The elastogenetic process in transplants and cultures of isolated auricular chondrocytes. Connect Tissue Res 8:171–181

    CAS  PubMed  Google Scholar 

  25. Gay S (1983) Immunology of collagen. In: Wagner BM, Fleischmajer RF (eds) Connective tissue and diseases of connective tissue. IAP Monograph. Williams and Wilkins, Baltimore pp 120–128

    Google Scholar 

  26. Poole AR, Pidoux I, Reiner A, Tangl H, Choi H, Rosenberg L (1980) Localization of proteoglycan monomer and link protein in the matrix of bovine articular cartilage: an immunohistochemical study. J Histochem Cytochem 28:621–635

    CAS  PubMed  Google Scholar 

  27. Sokoloff L (1980) In vitro culture of joints and articular tissues. In: Sokoloff L (ed) The joints and synovial fluid, vol 2. Academic Press, New York, pp 1–26

    Google Scholar 

  28. Srivastava VML, Malemud CJ, Sokoloff L (1974) Chondroid expression by lapine articular chondrocytes in spinner culture following monolayer growth. Connect Tissue Res 2:127–136

    Article  CAS  PubMed  Google Scholar 

  29. Deshmukh K, Kline WG (1976) Characterization of collagen and its precursors synthesized by rabbit articular cartilage cells in various culture systems. Eur J Biochem 69:117–123

    Article  CAS  PubMed  Google Scholar 

  30. Norby DP, Malemud CJ, Sokoloff L (1977) Differences in the collagen types synthesized by lapine articular chondrocytes in spinner and monolayer culture. Arthritis Rheum 20:709–716

    CAS  PubMed  Google Scholar 

  31. Benya PD, Shaffer JD (1982) Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30:215–224

    Article  CAS  PubMed  Google Scholar 

  32. Hsu TC, Benirschke K (1967) The atlas of mammalian chromosomes, vol 1. Springer-Verlag, New York

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA

    Jack M. Lipman, Cahir A. McDevitt & Leon Sokoloff

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  1. Jack M. Lipman
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  2. Cahir A. McDevitt
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  3. Leon Sokoloff
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Lipman, J.M., McDevitt, C.A. & Sokoloff, L. Xenografts of articular chondrocytes in the nude mouse. Calcif Tissue Int 35, 767–772 (1983). https://doi.org/10.1007/BF02405121

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