Summary
Fluorescein isothiocyanate (FITC) labeled lectins were used to study the distribution of specific binding sites in histologic sections of mouse ovaries as well as ovulated ova. Four distinct patterns of reactivity of the components of the follicle (exclusive of the ovum) and the surrounding ovarian stroma were recognized: 1. uniform staining of granulosa cells, theca cells and surrounding stroma; 2. weak to moderate staining of the granulosa cells and strong staining of the theca cells and stromal cells; 3. no reactivity of the granulosa cells coupled with strong reactivity of the theca and stromal cells; 4. no reactivity with any component of thecumulus oophorus. Three lectins (from Triticum vulgare, Arachis hypogaea and Maclura pomifera) distinctly accentuated the basal lamina of thecumulus oophorus. The reaction of lectins with oocytes and zona pellucida revealed six distinct patterns: 1. no reactivity with either structure; 2. weak reactivity with the cytoplasm of the oocyte and no reactivity with the zona pellucida; 3. very strong reactivity with the cytoplasm of the oocyte and no reactivity with the zona pellucida; 4. moderate reactivity with both the oocyte and the zona pellucida; 5. moderate reactivity with the oocyte and very strong reactivity with the zona pellucida; 6. no reactivity with the oocyte and moderate reactivity with the zona pellucida. The same pattern of reactivity was seen in the ovulated ova in the oviduct except for the lectin from Arachis hypogaea, the reactivity of which changed upon ovulation and/or fertilization of the ovum. These data provide a list of lectin markers for distinct components of the mouse ovary.
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References
Bagavandoss P, Midgley AR Jr, Wicha M (1983) Developmental changes in the ovarian follicular basal lamina detected by immunofluorescence and electron microscopy. J Histochem Cytochem 31:633–640
Fujimoto H, Muramatsu T, Urushihara H, Yanagisawa KO (1982) Receptors toDolichos biflorus agglutinin. A new cell surface marker common to teratocarcinoma cells and preimplantation mouse embryons. Differentiation 22:59–61
Goldstein IJ, Hayes CC (1978) The lectins: Carbohydrate-binding proteins. Adv Carbohydr Chem Biochem 35:127–340
Holthöfer H, Virtanen I, Pettersson E, Tornroth T, Alfthlan O, Linder E, Miettinen A (1981) Lectins as fluorescence microscopic markers for saccharides in the human kidney. Lab Invest 45:391–393
Holthöfer H, Virtanen J, Kariniemi A-L, Hormia M, Linder E, Miettinen A (1982)Ulex europeus I lectin as a marker for vasculer endothelium in human tissues. Lab Invest 47:60–66
Koehler JK (1980) Lectins as probes of the spermatozoon surface. Arch Androl 6:197–217
Lee M-C, Wu T-C, Wan Y-J, Damjanov I (1983) Pregnancy-related changes in the mouse oviduct and uterus revcaled by differential binding of fluoresceinated lectins. Histochemistry 79:365–375
Mazzuca M, Lhermitte M, Lafitte JJ, Roussel P (1982) Use of lectins for detection of glyconjugates in the glandular cells of the human bronchial mucosa. J Histochem Cytochem 30:956–966
Murata F, Tsuyama S, Suzuki S, Hamada H, Ozawa M, Muramatsu T (1983) Distribution of conjugates in the kidney, studied by use of labeled lectins. J Histochem Cytochem 31:139–144
Penna SD, Gordon BB, Karpati G, Carpenter S (1981) Lectin histochemistry of human skeletal muscle. J Histochem Cytochem 29:542–546
Ree HJ, Hsu S-M (1983) Lectin histochemistry of malignant tumors. I. Peanut agglutinin (PNA) receptors in follicular lymphoma and follicular hyperplasia: An immunohistochemical study. Cancer (Phila) 51:1631–1638
Schulte BA, Spicer SS (1983) Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. I. Mouse. Histochem J 15:1217–1238
Spicer SS, Schulte BA (1982) Identification of cell surface constituents. Lab Invest 47:2–4
Spicer SS, Baron DA, Sato A, Schulte BA (1981) Variability of cell surface glycoconjugates: relation to differences in cell function. J Histochem Cytochem 29:994–998
Watanabe M, Muramatsu T, Shirane H, Ugai K (1981) Discrete distribution of binding sites forDolichos biflorus agglutinin (DBA) and for peanut agglutinin (PNA) in mouse organ tissues. J Histochem Cytochem 29:779–790
Whyte A, Loke YW, Stoddart RW (1978) Saccharide distribution in human trophoblast demonstrated using fluorescein-labeled lectins. Histochem J 10:417–423
Wu T-C, Wan Y-J, Damjanov I (1983a) Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice. Histochemistry 77:233–241
Wu T-C, Wan Y-J, Damjanov I (1983b) Fluorescein conjugated Bandeiraea simplicifolia lectin as a marker of endodermal, yolk sac and trophoblastic differentiation in the mouse. Differentiation 24:55–59
Yonezawa S, Nakamura T, Tanaka S, Sato E (1982) Glycoconjugate withUlex europaeus agglutinin-I-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel. J Natl Cancer Inst 69:777–785
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Wu, T.C., Lee, M.C., Wan, Y.J. et al. Lectin binding sites of the mouse ovary, intraovarian and ovulated ova. Histochemistry 80, 527–533 (1984). https://doi.org/10.1007/BF02400967
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DOI: https://doi.org/10.1007/BF02400967