Abstract
We undertook a study to explore the potential of microsatellite loci as genetic markers for investigating kinship patterns in a social waspParachartergus colobopterus. A plasmid library with small inserts was screened for several oligonucleotide repeat motifs. Positive clones were sequenced and several were selected for further work. PCR primers were constructed that would amplify the tandem repeat region and a number of female wasps were scored for variation in the number of tandem repeats at these loci. The five amplified loci were far more variable than allozymes, with an average heterozygosity of 0.35.
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References
Ausubel, F. A., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds.) (1992).Current Protocols in Molecular Biology, Greene and Wiley-Interscience, New York.
Budowle, B., Giusti, A. M., Waye, J. S., Baechtel, F. S., Fourney, R. M., Adams, D. E., Presley, L. A., Deadman, H. A., and Monson, K. L. (1991). Fixed-bin analysis for statistical evaluation of continuous distributions of allelic data from VNTR loci, for use in forensic comparisons.Am. J. Hum. Genet. 48841.
Cornall, R. J., Aitman, T. J., Hearne, C. T., and Todd, J. A. (1991). The generation of a library of PCR-analyzed microsatellite variants for genetic mapping of the mouse genome.Genomics 10874.
Dietrich, W., Katz, H., Lincoln, S. E., Shin, H.-S., Friedman, J., Dracopoli, N. C., and Lander, E. S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses.Genetics 131423.
Edwards, A., Civitello, A., Hammond, H. A., and Caskey, C. T. (1991). DNA typing a genetic mapping with trimeric and tetrameric repeats.Am. J. Hum. Genet. 49746.
Edwards, A., Hammond, H., Jin, L., Caskey, C. T., and Chakraborty, R. (1992). Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups.Genomics 12241.
Gadagkar, R. (1991). On testing the role of genetic asymmetries created by haplodiploidy in the evolution of eusociality in the Hymenoptera.J. Genet. 701.
Graur, D. (1985). Gene diversity in Hymenoptera.Evolution 39190.
Gustincich, S., Manfioletti, G., Del Sal, G., Schneider, C., and Carnici, P. (1991). A fast method for high-quality DNA extraction from whole human blood.Biotechniques 11298.
Hamada, H., Petrino, M. G., and Kakunaga, T. (1982). A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes.Proc. Natl. Acad. Sci. 796465.
Hamilton, W. D. (1964a). The genetical evolution of social behaviour. I.J. Theor. Biol. 71.
Hamilton, W. D. (1964b). The genetical evolution of social behaviour. II.J. Theor. Biol. 717.
Hanotte, O., Bruford, M. W., and Burke, T. (1992). Multilocus DNA fingerprints in gallinaceous birds: General approach and problems.Heredity 68481.
Hearne, C. M., Ghosh, S., and Todd, J. A. (1992). Microsatellites for linkage analysis of genetic traits.Trends Genet. 8288.
Hudson, T. J., Engelstein, M., Matthias, K. L., Ho, E. C., Rubenfield, M. J., Adams, C. P., Housman, D. E., and Dracopoli, N. C. (1992). Isolation and chromosomal assignment of 100 highly informative human simple sequence repeat polymorphisms.Genomics 13622.
Hughes, C. R., and Queller, D. C. Detection of highly polymorphic microsatellite loci in a species with little enzyme polymorphism.Mol. Ecol. (in press).
Jeffreys, A. J., Wilson, V., and Thein, S. L. (1985). Hypervariable “minisatellite” regions in human DNA.Nature 31467.
Kwiatkowski, D. J., Henske, E. P., Weimer, K., Ozelius, L., Gusella, J. F., and Haines, J. (1992). Construction of a GT polymorphism map of human 9q.Genomics 12229.
Langridge, U., Schwall, M., and Langridge, P. (1991). Squashes of plant tissue as substrate for PCR.Nucleic Acids Res. 196954.
Litt, M., and Luty, J. A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.Am. J. Hum. Genet. 44397.
Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., and Erlich, H. (1986). Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction.Cold Spring Harbor Symp. Quant. Biol. 51263.
Nakamura, Y., Leppert, M., O'Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlin, E., and While, R. (1987). Variable number of tandem repeat (VNTR) markers for human genome mapping.Science 2351616.
Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals.Genetics 89583.
Pardue, M. L., Lowenhaupt, K., Rich, A., and Nordheim, A. (1987). (dC-dA)n · (dG-dT)n sequences have evolutionarily conserved chromosomal locations inDrosophila with implications for roles in chromosome structure and function.EMBO J. 61781.
Queller, D. C., and Goodnight, K. F. (1989). Estimating relatedness using genetic markers.Evolution 43258.
Queller, D. C., Strassmann, J. E., and Hughes, C. R. (1988). Genetic relatedness in colonies of tropical wasps with multiple queens.Science 2421155.
Rassmann, K., Schlötterer, C., and Tautz, D. (1991). Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting.Electrophoresis 12113.
Rosenberg, H. F., Ackerman, S. J., and Tenen, D. G. (1990). An alternative method for labeling oligonucleotide probes for screening cDNA libraries.Biotechniques 8384.
Saiki, R., Scharf, S., Faloona, F., Mullis, K., Horn, G., Erlich, H., and Arnheim, N. (1985). Enzymatic amplification of the beta globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.Science 2301350.
Sawadogo, M., and Van Dyke, M. W. (1991). A rapid method for the purification of deprotected oligodeoxynucleotides.Nucleic Acids Res. 19694.
Schlötterer, C., and Tautz, D. (1992). Slippage synthesis of simple sequence DNA.Nucleic Acids Res. 20211.
Serikawa, T., Kuramoto, T., Hilbert, P., Mori, M., Yamada, J., Dubay, C. J., Lindpainter, K., Ganten, D., Guénet, J.-L., Lathrop, G. M., and Beckmann, J. S. (1992). Rat gene mapping using PCR-analyzed microsatellites.Genetics 131701.
Stallings, R. L., Ford, A. F., Nelson, D., Torney, D. C., Hildebrand, C. E., and Moyzis, R. K. (1991). Evolution and distribution of (GT)n repetitive sequences in mammalian genomes.Genomics 10:807.
Strassmann, J. E., Hughes, C. R., Queller, D. C., Turillazzi, S., Cervo, R., Davis, S. K., and Goodnight, K. F. (1989). Genetic relatedness in primitively eusocial wasps.Nature 342268.
Strassmann, J. E., Queller, D. C., Solís, C. R., and Hughes, C. R. (1991). Relatedness and queen number in the neotropical wasp,Parachartergus colobopterus.Anim. Behav. 42461.
Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers.Nucleic Acids Res. 176463.
Tautz, D., and Renz, M. (1984). Simple sequences are ubiquitous repetitive components of eukaryotic genomes.Nucleic Acids Res. 124127.
Weber, J. L. (1990). Informativeness of human (dC-dA)n · (dG-dT)n polymorphisms.Genomics 7524.
Weber, J. L., and May, P. E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.Am. J. Hum. Genet. 44388.
Weissenbach, J., Gyapay, G., Dib, C., Vignal, A., Morissette, J., Millasseau, P., Vaysseix, G., and Lathrop, M. (1992). A second-generation linkage map of the human genome.Nature 359794.
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This work was supported by NSF Grants BSR-9021514 and IBN-9210051.
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Choudhary, M., Strassmann, J.E., Solís, C.R. et al. Microsatellite variation in a social insect. Biochem Genet 31, 87–96 (1993). https://doi.org/10.1007/BF02399822
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DOI: https://doi.org/10.1007/BF02399822