Abstract
An analysis of patterns of cleavage of mtDNA by restriction endonucleases was performed for nine individuals from the Philippine population of native cattle. MtDNA polymorphisms were detected in the restriction patterns generated by the following six enzymes,BamHI,BglII,EcoRV,HindIII,PstI, andScaI. The restriction patterns showing polymorphisms were distributed nonrandomly among the nine individuals examined from the Philippine population of native cattle, indicating the existence of two separate types of mtDNA. These two types of mtDNA are very different from each other, at the level of subspecies. Since the native Philippine cattle are considered to represent an admixture of European and Indian cattle, the two types of mtDNA must be derived from the mtDNAs of both varieties. The polymorphic sites in mtDNA have been located on a restriction map, and the nucleotide substitutions at some of the sites have also been estimated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, S., de Bruijn, M. H. L., Coulson, A. R., Eperon, I. C., Sanger, F., and Young, I. G. (1982). Complete sequence of bovine mitochondrial DNA: Conserved features of the mammalian mitochondrial genome.J. Mol. Biol. 156683.
Brown, W. M., George, M., Jr., and Wilson, A. C. (1979). Rapid evolution of animal mitochondrial DNA.Proc. Natl. Acad. Sci. USA 761967.
Brown, W. M., Prager, E. M., Wang, A., and Wilson, A. C. (1982). Mitochondrial DNA sequences of primates: Tempo and mode of evolution.J. Mol. Evol. 18225.
Ferris, S. D., Sage, R. D., Huang, C.-M., Nielsen, J. T., Ritte, U., and Wilson, A. C. (1983). Flow of mitochondrial DNA across a species boundary.Proc. Natl. Acad. Sci. USA 802290.
Namikawa, T. (1981). Geographical distribution of bovine hemoglobin-beta (Hbb) alleles and the phylogenetic analysis of the cattle in eastern Asia.Z. Tierzuchtg. Zuchtgsbiol. 98151.
Nei, M., and Li, W.-H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases.Proc. Natl. Acad. Sci. USA 765269.
Nei, M., and Tajima, F. (1983). Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data.Genetics 105207.
Wakana, S., Watanabe, T., Hayashi, Y., and Tomita, T. (1986). A variant in the restriction endonuclease cleavage pattern of mitochondrial DNA in the domestic fowls (Gallus gallus domesticus).Anim. Genet. 17159.
Watanabe, T., Hayashi, Y., Ogasawara, N., and Tomita, T. (1985a). Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns.Biochem. Genet. 23105.
Watanabe, T., Mizutani, M., Wakana, S., and Tomita, T. (1985b). A demonstration of maternal inheritance of avian mitochondrial DNA in chicken-quail hybrids.J. Exp. Zool. 236245.
Watanabe, T., Hayashi, Y., Semba, R., and Ogasawara, N. (1985c). Bovine mitochondrial DNA polymorphism in restriction endonuclease cleavage patterns and location of the polymorphic sites.Biochem. Genet. 23947.
Watanabe, T., Hayashi, Y., Kimura, J., Yasuda, Y., Saitou, N., Tomita, T., and Ogasawara, N. (1986). Pig mitochondrial DNA: Polymorphism, restriction map orientation and sequence data.Biochem. Genet. 24385.
Yonekawa, H., Moriwaki, K., Gotoh, O., Miyashita, N., Migita, S., Bonhomme, F., Hjorth, J. P., Petras, M. L., and Tagashira, Y. (1982). Origins of inbred mice deduced from restriction patterns of mitochondrial DNA.Differentiation 22222.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watanabe, T., Masangkay, J.S., Wakana, S. et al. Mitochondrial DNA polymorphism in native Philippine cattle based on restriction endonuclease cleavage patterns. Biochem Genet 27, 431–438 (1989). https://doi.org/10.1007/BF02399672
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02399672