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Cell kinetic study of the gallbladder epithelium, with special reference to the pancreaticobiliary maljunction

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Journal of Hepato-Biliary-Pancreatic Surgery

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Abstract

The bromodeoxyuridine labelling index (BrdU-LI), ornithine decarboxylase (ODC) activity, and bile elements in the gallbladder were examined in 23 patients to investigate cell kinetics and oncogenesis in gallbladder epithelium with pancreaticobiliary maljunction (PBMJ). The patients were classified into five groups: group I (4 patients without biliary disorder), group II (8 patients with cholecystolithiasis), group III (4 patients with PBMJ), group IV (3 patients with gallbladder cancer and PBMJ), and group V (4 patients with gallbladder cancer). In non-cancerous epithelium, the BrdU-LI in groups III (2.88±3.07%), IV (4.92±2.73), and V (5.93±2.08) was significantly higher than in group I (0.06±0.12), and the ODC activity in groups III (7.83±6.82 pmole CO2/h per mg protein), IV (17.21±9.44), and V (11.27±12.33) was also significantly higher than that in group I (1.39±1.78). Both BrdU-LI and ODC activity were high in epithelium showing metaplasia in 3 patients from groups III and IV. In groups I and II, the lithocholic acid (LCA) fraction showed a positive correlation with BrdU-LI (correlation coefficient, 0.90) and ODC activity (correlation coefficient, 0.91). From these results, it can be concluded that the gallbladder epithelium in patients with PBMJ, and its metaplastic changes, show an increase in cell proliferation, which is important in the progression of cancer.

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References

  1. De Bore WGRM, Ma J, Rees JW, Nayman J (1981) Inappropriate mucin production in gallbladder metaplasia and neoplasia — an immunohistological study. Histopathology 5: 295–303

    Google Scholar 

  2. Kozuka S, Kurashina M, Tsubone M, Hachisuka K, Yasui A (1984) Significance of intestinal metaplasia for the evolution of cancer in biliary tract. Cancer 54: 2277–2285

    CAS  PubMed  Google Scholar 

  3. Komi N, Tamura T, Miyoshi Y, Hino M, Yada S, Kawahara H, Udaka H, Takehara H (1985) Histochemical and immunohistochemical studies on development of biliary carcinoma in 47 patients with choledochal cyst — special reference to intestinal metaplasia in biliary duct. Jpn J Surg 15:273–278

    CAS  PubMed  Google Scholar 

  4. Funabiki T, Sugiue K, Matsubara T, Kamei K, Hasegawa S, Yamaguchi H, Ito H, Kato H, Kishikawa T, Toda T (1989) Biochemical and histopathological studies on anomalous arrangement of the pancreatobiliary ductal system with cancerous changes (in Japanese with English abstract). Nippon Syonigeka Gakkai Zasshi (J Jpn Soc Pediatr Surgeons) 25:58–64

    Google Scholar 

  5. Kato T, Matsuda K, Kayaba H, Enomoto S, Tamura H, Hebiguchi T, Koyama K, Hachiya N, Takizawa Y (1987) Pathological condition of anomalous choledocho-pancreatic ductal junction — mutagenicity of biliary tract contents and fluorometric analysis of DNA content of biliary epithelia (in Japanese with English abstract). Tando (J Jpn Bil Assoc) 1:377–383

    Google Scholar 

  6. Noguchi A (1988) Cell kinetic studies on mucosa of the gallbladder by using anti-bromodeoxyuridine monoclonal antibody in patients with anomalous arrangment of the pancreatobiliary ductal system (in Japanese). Nippon Syokakigeka Gakkai Zasshi (Jpn J Gastroenterol Surg) 21:210–220

    Google Scholar 

  7. Tokiwa K, Iwai N, Okuzumi J, Yamane T, Fujita Y, Takahashi T (1990) Ornithine decarboxylase activity in the mucosa of gallbladder in patients with anomalous arrangement of pancreaticobiliary duct (in Japanese with English abstract). Igakuno Ayumi (J Clin Exp Med) 152:193–194

    Google Scholar 

  8. The Japanese study group on pancreaticobiliary maljunction (JSPBM). The committee of diagnostic criteria (1991) Diagnostic criteria of pancreaticobiliary maljunction (in Japanese with English abstract). Nippon Suikantandogoryuijyo Kenkyukai Puroshidingu (Proc JSPBM) 14:7–12

    Google Scholar 

  9. Todani T, Watanabe Y, Narusue M, Tabuchi K, Okajima K (1977) Congenital bile duct cysts: Classification, operation procedure, and review of 37 cases, including cancer arising from choledochal cyst. Am J Surg 134:263–269

    Article  CAS  PubMed  Google Scholar 

  10. Morstyn G, Hsu SM, Kinsella T, Gratzner H, Russo A, Mitchell JB (1983) Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody. J Clin Invest 72:1844–1850

    Article  CAS  PubMed  Google Scholar 

  11. Fujiki H, Mori M, Nakayasu M, Terada M, Sugimura T (1979) A possible naturally occurring tumor promoter, teleocidin B from streptomyces. Biochem Biophys Res Commun 90:975–983

    Article  Google Scholar 

  12. Okuyama S (1981) Analysis of free, glycine- and taurine-conjugated individual bile acids using high performance liquid chromatography and immobilized 3α-hydroxysteroid, dehydrogenase in column form (in Japanese). Rinsyobyori (Jpn J Clin Pathol) 29:446–458

    CAS  Google Scholar 

  13. Maeda K, Iwai N, Okuda G, Kouketu H (1986) Measurement of α-amylase activity (in Japanese). Rinsyokensa kiki·Shiyaku (J Clin Labo Instruments and Reagents) 9:102–106

    Google Scholar 

  14. Iwachika C, Takeda M, Demura H, Shiratori K, Watanabe S, Takeuchi T (1984) Measurement of serum carcinoembryonic antigen (CEA) using a monoclonal antibody to CEA (in Japanese). Igaku to Rinsyou (J Med Pharmaceut Sci) 12: 125–133

    Google Scholar 

  15. Todani T, Tabuchi K, Watanabe Y, Kobayashi T (1979) Carcinoma arising in the wall of congenital bile duct cyst. Cancer 44:1134–1141

    CAS  PubMed  Google Scholar 

  16. Babbitt DP, Starshak RJ, Clemett AR (1973) Choledochal cyst: A concept of etiology. AJR 119:57–62

    CAS  Google Scholar 

  17. Konishi F (1987) Pathological aspects of high risk states developing to cancer of the extrahepatic biliary tracts (in Japanese). Tan to Sui (Bil Tract and Pancreas) 8:1493–1499

    Google Scholar 

  18. Shinozaki M, Kimura K, Ohto M (1988) Clinical features and treatment of anomalous pancreaticobiliary ductal union in adults (in Japanese). Tanto Sui (Bil Tract and Pancreas) 9:1165–1170

    Google Scholar 

  19. Aoki H, Sugatani H, Shimazu M (1987) A clinical study on cancer of the bile duct associated with anomalous arrangement of pancreaticobiliary ductal system. Analysis of 569 cases collected in Japan (in Japanese). Tanto Sui (Bil Tract and Pancreas) 8:1539–1551

    Google Scholar 

  20. Bleiberg H, Salhadin A, Galand P (1977) Cell cycle parameters in human colon — comparison between primary and recurrent adenocarcinomas, benign polyps, and adjacent unaffected mucosa. Cancer 39:1190–1194

    CAS  PubMed  Google Scholar 

  21. Westin T, Edstrom S, Lundholm K, Gustafsson B (1991) Evaluation of ornithine decarboxylase activity as a marker for tumor growth rate in malignant tumors. Am J Surg 162:288–293

    Article  CAS  PubMed  Google Scholar 

  22. Moshier JA, Dosescu J, Skunca M, Luk GD (1993) Transformation of NIH/3T3 cells by ornithine decarboxylase overexpression. Cancer Res 53:2618–2622

    CAS  PubMed  Google Scholar 

  23. Narisawa T, Magadia NE, Weisburger JH, Wynder EL (1974) Promoting effect of bile acid on colon carcinogenesis after intrarectal instillation of N-Methyl-N′-nitro-N-nitrosoguanidine in rats. J Natl Cancer Inst 53:1093–1097

    CAS  PubMed  Google Scholar 

  24. Morita S (1990) Studies on the promoting effect of lithocholic acid on cholangiocarcinoma induced by di-isopropanol nitrosoamine (DIPN) in hamsters (in Japanese with English abstract). Tando (J Jpn Bil Assoc) 4:430–442

    Google Scholar 

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Authors and Affiliations

  1. The First Department of Surgery, Fukui Medical School, 23 Shimoaizuki, Matsuoka, 910-11, Yoshida, Fukui, Japan

    Takashi Fujita & Gizo Nakagawara

  2. The Second Department of Surgery, Yokohama City University School of Medicine, 3-9 Fukura, 236, Kanazawa, Yokohama, Japan

    Hiroshi Shimada

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  1. Takashi Fujita
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  2. Hiroshi Shimada
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  3. Gizo Nakagawara
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Fujita, T., Shimada, H. & Nakagawara, G. Cell kinetic study of the gallbladder epithelium, with special reference to the pancreaticobiliary maljunction. J Hep Bil Pancr Surg 1, 229–235 (1994). https://doi.org/10.1007/BF02391072

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  • DOI: https://doi.org/10.1007/BF02391072

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