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Intact organ culture of murine metanephros

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Journal of tissue culture methods

Summary

A murine metanephric whole-organ culture system is described which maintains normal structural relationships of epithelial and mesenchymal elements and undergoes advanced organotypic differentiation in vitro. Manipulation of the culture conditions permits study of the developmental, immunologic, and biochemical aspects of normal and abnormal renal organogenesis.

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Authors and Affiliations

  1. Division of Pediatric Nephrology, Children's Hospital and Medical Center, and the University of Washington, 98105, Seattle, Washington

    William E. Sweeney Jr. & Ellis D. Avner

Authors
  1. William E. Sweeney Jr.
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  2. Ellis D. Avner
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Sweeney, W.E., Avner, E.D. Intact organ culture of murine metanephros. Journal of Tissue Culture Methods 13, 163–168 (1991). https://doi.org/10.1007/BF02388120

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  • DOI: https://doi.org/10.1007/BF02388120

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