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Effect of IL-6 on tumor cell invasion of vascular endothelial monolayers

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Abstract

The effect of interleukin-6 (IL-6) on the invasive capacity of B16-F1 mouse melanoma cells into vascular endothelial monolayers was examined, and an in vitro assay system for the quantitative determination of tumor cell invasiveness, using confocal microscopy with a fluorescence image analyzer, was developed. First, the invasive capacity of B16-F1 mouse melanoma cells against bovine vascular endothelial monolayers was estimated; then, the gap junctional intercellular communication (GJIC) of endothelial cells was examined. Treatment of endothelial cells with IL-6 resulted in a remarkable increase in the invasion of tumor cells into the endothelial monolayer, which was found to be significant from 25 ng/ml, and peaked at levels of more than 50 ng/ml. This stimulatory effect of IL-6, which was observed from 3 h after the initiation of treatment and lasted for up to 24 h, was abolished by the addition of the anti-IL-6 antibody. Although phasecontrast microscopy did not reveal any morphological changes in the endothelial cells following treatment with 25–200 ng/ml IL-6 for 24h, the GJIC was observed to be significantly decreased. These findings indicate that the invasive capacity of tumor cells into endothelial cells is affected by IL-6.

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Authors and Affiliations

  1. Department of Physiological Chemistry, School of Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113, Tokyo, Japan

    Yoshiaki Kitamura, Ikuo Morita & Sei-Itsu Murota

  2. Second Department of Surgery, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113, Tokyo, Japan

    Yoshiaki Kitamura, Zenro Nihei & Yoshio Mishima

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  1. Yoshiaki Kitamura
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  2. Ikuo Morita
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  3. Zenro Nihei
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  4. Yoshio Mishima
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  5. Sei-Itsu Murota
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Kitamura, Y., Morita, I., Nihei, Z. et al. Effect of IL-6 on tumor cell invasion of vascular endothelial monolayers. Surg Today 27, 534–541 (1997). https://doi.org/10.1007/BF02385807

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  • DOI: https://doi.org/10.1007/BF02385807

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