Abstract
A method for maintaining anin vivo culture ofEntomophthora muscae (C) Fres. on its original host, adult carrot flies (Psila rosae F.), is described. The lethal time for adult carrot flies was greatly influenced by temperature, both for infected and for uninfected flies. In the range 8.2°C–20.2°C the LT50 for infected flies was about 5.4 times shorter than the estimated average life-span for uninfected flies. The discharge of primary spores was also strongly dependent on temperature. The total number of primary spores discharged per fly at 100% RH and in darkness ranged between 1.2×104 and 9.6×104 with a mean of 5.1×104.
Zusammenfassung
Eine Methode für einein vivo Kultur vonE. muscae wird beschrieben. Die Nahrung der Pilzkultur bestand aus ihrem natürlichen Wirt, erwachsene Möhrenfliegen (P. rosae). Die Lebensdauer der erwachsenen Möhrenfliegen hängt im hohen Mass von der Temperatur ab, sowohl was infizierte als uninfizierte Fliegen betrifft. Im Interval von 8.2°C–20.2°C war die LT50 für infizierte Fliegen etwa 5.4 mal kürzer als die durchschnittliche Lebensdauer (“LT50”) für uninfizierte Fliegen. Das Abschleuderung der Primärsporen zeigte auch eine starke Temperaturabhängigkeit. Die totale Anzahl von Primärsporen pro Fliege in 100% RH und Dunkelheit abgeschleudert, lag zwischen 1.2×104 und 9.6×104 und Betrug im Mittel 5.1×104.
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Eilenberg, J. The culture ofEntomophthora muscae (C) Fres. in carrot flies (Psila rosae F.) and the effect of temperature on the pathology of the fungus. Entomophaga 32, 425–435 (1987). https://doi.org/10.1007/BF02372452
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DOI: https://doi.org/10.1007/BF02372452