Abstract
The influence ofKlebsiella oxytoca andEnterobacter cloacae inoculation on dinitrogen fixation by the rice-bacteria association was examined in pots in a greenhouse. For inoculation,K. oxytoca NG13 isolated from a Japan paddy soil,E. cloacae E26 isolated from a China soil and following three strains were employed.K. oxytoca NG1389 is a mutant from NG13 and has no nitrogenase activity (nif). K. oxytoca NG13/pMC71A andE. cloacae E26/pMC71A were produced by inserting anif A containingK. pneumoniae plasmid (pMC71A) to NG13 and E26, respectively. These two strains were able to fix dinitrogen fixation in the presence of ammonium, whereas nitrogenase activity of wild strains (NG13 and E26) were repressed under this condition.
Inoculation effects were tested on two rice (Oryza sativa L.) varieties, a Indica type C5444 and a Japonica type T65. Rice seedlings were planted to nonsterilized potted soil, and grown under flooded conditions. Upon inoculation with NG13 and E26, growth and N increment of plants particularly in T65 were stimulated above NG1389 inoculated plants. Assay by15N dilution and acetylene reduction techniques indicated that this N increment may be due to fixed N. Inoculation with NG13/pMC71A and E26/pMC71A resulted in more dry weight and fixed N than those of NG13 and E26 inoculated plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adelberg E A, Mandel M and Chen G C C 1965 Optimal conditions for mutagenesis by N-methyl-N-nitro-N-nitrosoguanidine inEscherichia coli K12. Biochem. Biophys. Res. Comm. 18, 788–795.
App A A, Watanabe I, Alexander M, Ventura W, Daez C, Santiago T and De Datta S K 1980 Nonsymbiotic nitrogen fixation associated with the rice plant in flooded soil. Soil Sci. 130, 283–289.
Balandreau J, Rinaudo G, Fres-Hamad I and Dommergues 1975 Nitrogen fixation in the rhizosphere of rice plants.In Nitrogen Fixation by Free Living Microorganisms. Ed. W D P Stewart. pp. 53–70. Cambridge Univ. Press, Cambridge.
Bally R, Thomas-Bauzon D, Heulin TH, Balandreau J, Richard C, and De Ley J 1983 Determination of the most frequent N2-fixing bacteria in a rice rhizosphere. Can. J. Microbiol 29, 881–887.
Buchanan-Wollaston V, Cannon M C, Beynon J L, and Cannon F C 1981 Role of thenifA gene product in the regulation ofnif expression inKlebsiella pneumoniae. Nature (London) 294, 776–778.
Davis B D and Mingioli E S 1950 Mutant ofEscherichia coli requiring methionine or vitamine B12. J. Bacteriol. 60, 17–28.
Dixon R A, Eady R R, Espin G, Hill S, Iaccarino M, Kahn D and Merrick M 1980 Analysis of regulation ofKlebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusion. Nature (London) 286, 128–132.
Hirota Y, Fujii T, Sano T and Iyama S (1978) Nitrogen fixation in the rhizosphere of rice. Nature (London) 276, 416–417.
Kumari M L, Kavimandan S K and Subba Rao N S 1976 Occurrence of nitrogen fixing Spirillum in roots of rice, sorghum, maize and other plants. Indian J. Exp. Biol. 14, 638–639.
Lahda J K, Barraquio W L and Watanabe I 1983 Isolation and identification of nitrogen-fixingEnterobacter cloacae andKlebsiella planticola associated with rice plants. Can. J. Microbiol. 29, 1301–1308.
Lederberg E M, and Cohen S N 1974 Transformation ofSalmonella typhimurium by plasmid deoxyribonucleic acid. J. Bacteriol. 119, 1072–1074.
Maniatis T, Fritsch E F and Sambrook J 1982 Molecular Cloning. Cold Spring Harbor Laboratory.
Qui Y S, Zhou S, Mo X, Ye S, Cai, Chen C Y, He S, and Deng R 1981 Study of nitrogen fixing bacteria associated with rice root 11. The characteristics of nitrogen fixation byAlcaligenes faecalis strain A-15 andEnterobacter cloacae E26. Acta Microbiol. Sinica 21, 473–476.
Rao V R, Nayak D N, Charyulu P B B N and Adhya T K 1983 Yield response of rice to root inoculation with Azospirillum. J. Agric. Sci. Camb. 100, 689–691.
Sano Y, Fujii T, Iyama S, Hirota Y and Komagata K 1981 Nitrogen fixation in the rhizosphere of cultivated and wild rice strains. Crop Sci. 21, 758–761.
Subba Rao N S, Tilak K V B R, Singh C S and Lakshmikumari M 1979 Response of a few economic species of graminaceous plants to inoculation withAzospirillum lipoferum. Curr. Sci. 48, 133–134.
Thomas-Bauzon D, Weinhard P, Villecourt P and Balandreau J 1982 The spermoshere model I. Its use in growing, counting, and isolating N2-fixing bacteria from the rhizosphere of rice. Can. J. Microbiol. 28, 922–928.
Watanabe I and Lin C 1984 Response of wetland rice to inoculation withAzospirillum lipoferum andPseudomonas sp. Soil Sci. Plant Nutr. (Tokyo) 30, 117–124.
Watanabe I and Roger P A 1984 Nitrogen fixation in wetland rice field.In Current Developments in Biological Nitrogen Fixation. Ed. N S Subba Rao. pp 237–276. Oxford and IBH Publishing co. New Delhi.
Yoo I D, Fujii T, Sano Y, Komagata K, Yoneyama T, Iyama S, and Hirota Y 1986 Dinitrogen fixation of rice-Kebsiella association. Crop Sci. 26, 297–301.
Zhu J B, Yu G Q, Jiang Y Q, Wang L W and Shen S C 1983 Effect ofnif A product on suppression ofnif phenotype ofgln mutation and constitutive synthesis of nitrogenase inKlebsiella pneumoniae. Scientia Sinica (Series B), 1258–1268.
Author information
Authors and Affiliations
Additional information
Dr Y. Hirota died on 23 December 1986.
Rights and permissions
About this article
Cite this article
Fujii, T., Huang, YD., Higashitani, A. et al. Effect of inoculation withKlebsiella oxytoca andEnterobacter cloacae on dinitrogen fixation by rice-bacteria associations. Plant Soil 103, 221–226 (1987). https://doi.org/10.1007/BF02370392
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02370392