Abstract
This paper describes a new, accurate method for determination of glucose in soil enzyme assays. The low limit of detection of glucose by the peroxidase-glucose oxidase (PGO) system is 0.004 mg glucose · g soil−1. Comparisons of PGO with the anthrone and phenol-sulfuric acid methods revealed no differences in the detection of glucose standard solutions. By eliminating the use of large volumes of H2SO4, the PGO system reduces laboratory hazards.
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A portion of this work was supported by National Science Foundation Grant DEB-8004172 and a grant from the Miami University Faculty Research Committee.
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Kapustka, L.A., Annala, A.E. & Swanson, W.C. The peroxidase-glucose oxidase system: A new method to determine glucose liberated by carbohydrate degrading soil enzymes. Plant Soil 63, 487–490 (1981). https://doi.org/10.1007/BF02370048
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DOI: https://doi.org/10.1007/BF02370048