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A laboratory method for measuring the degree of attack byPhytophthora infestans

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Summary

A method is described based on quantitative measurements of infection efficiency (IE), lesion growth (LES) and conidia production (CON). The three parameters have been used to calculate the leaf area destroyed by blight in order to illustrate varietal differences in disease progress and to correlate the findings with field assessments. The method has also been used as a replacement or supplement of field readings in experiments designed for studies of fungicidal treatments.

Zusammenfassung

Es wird eine Methode beschrieben, die auf quantitativen Messungen des Infektionserfolges (IE), des Läsionenwachstums (LES) und der Konidienproduktion (CON) beruht. Um die Methode zu testen, wurden Blattmuster aus Feldversuchen genommen, die zum Studium von Fungizidbehandlungen (Tabellen 1 und 2) und der Resistenz von Kartoffelsorten gegen Krautfäule (Tabellen 3–7) angelegt wurden.

Die Methode ist genügend genau, um selbst kleine Unterschiede zu erfassen, die mit den bei Feldbeurteilungen angewendeten subjektiven Bewertungsmethoden nach Punkten schwerlich festgestellt werden. Die Daten der drei Parameter wurden zur Errechnung der durch Krautfäule zerstörten Blattfläche verwendet, um die sortenbedingten Unterschiede im Krankheitsverlauf zu illustrieren und die Ergebnisse mit den Feldbeurteilungen zu korrelieren (Tabellen 8, 9 und Abb. 4).

Résumé

La méthode décrite est basée sur une mesure quantitative du taux de contamination (IE =infection efficiency), des lésions en végétation (LES) et de la production de conidies (CON).

Pour tester la méthode, des échantillons de feuilles ont été prélevés dans les parcelles d'essais mis en place pour l'étude de fongicides (tableaux 1 et 2) et pour l'étude de la résistance au mildiou de diverses variétés (tableaux 3–7).

La technique est suffisamment précise pour détecter de faibles différences, difficiles à établir par une méthode de notation subjective utilisée pour les évaluations au champ.

Les données de trois paramètres ont été utilisées pour calculer la surface foliaire détruite par le mildiou, afin de mettre en évidence des différences variétales dans la progression de la maladie et de mettre en corrélation les résultats et l'évaluation au champ (tableaux 8 et 9. fig. 4).

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Author notes

  1. D. I. Ihnell

    Present address: The National Swedish Plant Protection Institute, S-171 07, Solna 7, Sweden

Authors and Affiliations

  1. The Swedish Seed Association, S-268 00, Svalov, Sweden

    V. Umaerus

Authors
  1. V. Umaerus
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  2. D. I. Ihnell
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Additional information

Communication from the Swedish Seed Association, No 422.

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Umaerus, V., Ihnell, D.I. A laboratory method for measuring the degree of attack byPhytophthora infestans . Potato Res 19, 91–107 (1976). https://doi.org/10.1007/BF02361997

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  • DOI: https://doi.org/10.1007/BF02361997

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