Summary
A method has been developed for the rapid quantification of glucose, fructose and sucrose in potato tubers by capillary gas chromatography. Baseline separation of trimethylsilyl derivatives of β-D(−) fructose, α-D(+) glucose β-D(+) glucose, myoinositol (internal standard) and sucrose was achieved in under 10 min with a detection range of 10 ng to 3 μg per component. Use of an on-column injector resulted in the retention of a proportion of injected sucrose by the column, despite an oven starting temperature of 140°C. This did not occur with a splitless injection system (inlet temperature 220°C).
This is a preview of subscription content, log in via an institution to check access.
References
Baijal, B. D. & W. F. van Vliet, 1966. The chemical composition in different parts of the potato tuber during storage.European Potato Journal 9: 179–190.
Binder, H., 1980. Separation of monosaccharides by high performance liquid chromatography: comparison of ultraviolet and refractive index detection.Journal of Chromatography 189: 414–420.
Benedict, S. R. & E. Osterberg, 1918. Determination of sugar in normal urine.Journal of Biological Chemistry 34: 195–201.
Cerning-Beroard, M., 1975. A note on sugar determination by the anthrone method.Cereal Chemistry 52: 857–860.
Cronin, D. A. & S. Smith, 1979. A simple and rapid procedure for the analysis of reducing, total and individual sugars in potatoes.Potato Research 22: 99–105.
Jones, M. G. K., W. H. Outlaw & O. H. Lowry, 1977. Enzymic assay of 10−7 to 10−4 moles of sucrose in plant tissues.Plant Physiology 60: 379–383.
Heatherbell, D. A., 1974. Rapid concurrent analysis of fruit sugars and acids by gas-liquid chromatography.Journal of the Science of Food and Agriculture 25: 1095–1107.
Holligan, P. M. & E. A. Drew, 1971. Routine analysis by gas-liquid chromatography of soluble carbohydrates in extracts of plant tissues. 2. Quantitative analysis of standard carbohydrates, and the separation and estimation of soluble sugars and polyols from a variety of plant tissues.New Phytologist 70: 271–297.
Lawson, M. A. & G. F. Russell, 1980. Trace level analysis of reducing sugars by high performance liquid chromatography.Journal of Food Science 45: 1256–1258.
Lindsay, H., 1973. A colorimetric estimation of reducing sugars in potatoes with 3,5-dinitrosalicylic acid.Potato Research 16: 176–179.
Sweeley, C. C., R. Bentley, M. Makita & W. W. Wells, 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances.Journal of the American Chemical Society 85: 2497–2507.
Varns, J. L. & R. Shaw, 1973. An internal standard for rapid analysis of potato sugars by gas chromatography.Potato Research 16: 183–187.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davies, H.V. Rapid determination of glucose, fructose and sucrose in potato tubers by capillary gas chromatography. Potato Res 31, 569–572 (1988). https://doi.org/10.1007/BF02361846
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02361846