Skip to main content
SpringerLink
Log in

Resistance to late blight (Phytophthora infestans (Mont.) de Bary) of potato plants regenerated fromin vitro selected calli

  • Published:
Potato Research Aims and scope Submit manuscript

Summary

Cells of seven potato cultivars were selectedin vitro with culture filtrate (CF) ofPhytophthora infestans (Mont.) de Bary. Regenerated plants were tested for late blight resistance. The aim of the study was to check the efficacy of CF in the selection of potatoes for resistance to late blight and to evaluate the effects of additive factors on general resistance. One selection cycle, applied to a cell culture system, selected cells resistant to toxic metabolites of CF.In vivo screening of clones regenerated from selected cells was done in two steps: on whole plants, assessing foliage late blight, and on detached leaves, assessing single factors of horizontal resistance.

In general, the frequency of resistant variants selected with CF did not differ from that of resistant somaclonal variants. Nevertheless, high concentrations of CF in the growth medium seemed to induce an improvement of some partial resistance factors as compared with the source plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alicchio, R., C. Antonioli, L. Graziani, R. Roncarati & C. Vannini, 1987. Isoenzyme variation in leaf-callus regenerated plants ofSolanum tuberosum.Plant Science 53: 31–86.

    Google Scholar 

  • Alicchio, R., 1990. Somaclonal variation inSolanum melongena. In: Y. P. S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry. Vol. 11. Somaclonal variation in crop improvement I. pp. 416–434. Springer-Verlag, Berlin, Heilderberg.

    Google Scholar 

  • Behnke, M., 1979. Selection of potato callus for resistance to culture filtrates ofPhytophthora infestans and regeneration of resistant plants.Theoretical and Applied Genetics 55: 69–71.

    Article  Google Scholar 

  • Behnke, M., 1980. General resistance to late blight ofSolanum tuberosum plants regenerated from callus resistant to culture filtrates ofPhytophthora infestans.Theoretical and Applied Genetics 56: 151–152.

    Article  Google Scholar 

  • Breiman, A. & I. Barash, 1981. Partial characterization of phytotoxic compounds in culture filtrates ofPhytophthora citrophthora.Phytopathologische Zeitschrift 102: 1–9.

    CAS  Google Scholar 

  • Brettell, I. R. S., E. Thoma & D. S. Ingram, 1980. Reversion of Texas male-sterile cytoplasm maize in culture to give fertile T-toxin resistant plants.Theoretical and Applied Genetics 58: 55–58.

    Google Scholar 

  • Chawla, H. S. & G. Wenzel, 1987.In vitro selection of barley and wheat for resistance againstHelminthosporium sativum.Theoretical and Applied Genetics 74: 841–845.

    Article  Google Scholar 

  • Foroughi-Wehr, B. & K. Stolle, 1985. Resistenzselektionin vitro am Beispiel des Systems Kartoffel/Phytophthora infestans (Mont.) de Bary.Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 37: 170–173.

    Google Scholar 

  • Gengenbach, B. G., C. E. Green & C. M. Donovan, 1977. Inheritance of selected pathotoxin resistance in maize plants regenerated from cell culture.Proceedings of the National Academy of Science of the United States of America 74: 5113–5117.

    CAS  Google Scholar 

  • Henniger, H., 1959. Versuche zur Kultur verschiedener Rassen vonPhytophthora infestans (Mont.) de Bary auf künstlichen Nährböden.Phytopathologische Zeitschrift 34: 285–306.

    Google Scholar 

  • Larkin, P. J. & W. R. Scowcroft, 1983. Somaclonal variation and eyespot toxin tolerance in sugarcane.Plant Cell Tissue and Organ Culture 2: 111–121.

    Article  CAS  Google Scholar 

  • Latunde-Dada, A. O. & J. A. Lucas, 1983. Somaclonal variation and reaction toVerticillium wilt inMedicago sativa L. plants regenerated from protoplasts.Plant Science Letters 32: 205–211.

    Article  Google Scholar 

  • Ling, D. H., P. Vidhyaseharan, E. S. Borromeo, F. J. Zapata & T. W. Mew, 1985.In vitro screening of rice germoplasm for resistance to brown spot disease using phytotoxin.Theoretical and Applied Genetics 71: 133–135.

    Article  CAS  Google Scholar 

  • MacDonald, M. V. & D. S. Ingram, 1985.In vitro selection for resistance toAlternaria brassicicola inBrassica napus ssp.oleifera (winter oilseed rape) using partially purified culture filtrates.Cruciferae Newsletter 10: 97–100.

    Google Scholar 

  • MacDonald, M. V. & D. S. Ingram, 1986. Towards the selectionin vitro for resistance toAlternaria brassicicola (Schw.) Wilts. inBrassica napus ssp.oleifera (Metzg.) Sinsk., winter oilseed rape.New Phytologist 104: 621–629.

    Google Scholar 

  • Malcomson, J. F., 1976. Assessment of field resistance to blight (Phytophthora infestans) in potatoes.Transactions of the British Mycological Society 67: 321–325.

    Google Scholar 

  • Parlevliet, J. E. & J. C. Zadoks, 1977. The integrated concept of disease resistance; a new view including horizontal and vertical resistance in plants.Euphytica 26: 5–21.

    Google Scholar 

  • Rines, H. W. & H. H. Luke, 1985. Selection and regeneration of toxin-insensitive plants from tissue cultures of oats (Avena sativa) susceptible toHelminthosporium victoriae.Theoretical and Applied Genetics 71: 16–21.

    Article  Google Scholar 

  • Sacristán, M. D., 1982. Resistance responses toPhoma lingam of plants regenerated from selected cell and embryogenic cultures of haploidBrassica napus.Theoretical and Applied Genetics 61: 193–200.

    Google Scholar 

  • Sacristán, M. D., 1985. Selection for disease resistance inBrassica cultures.Hereditas Supplement 3: 57–63.

    Google Scholar 

  • Scala, A., P. Bettini, M. Buiatti, P. Bogani, G. Pellegrini & F. Tognoni, 1985. Tomato-Fusarium oxysporum f.sp.lycopersici interaction:in vitro analysis of several possible pathogenic factors.Phytopathologische Zeitschrift 113: 90–94.

    Google Scholar 

  • Shahin, E. A. & R. Spivey, 1986. A single dominant gene forFusarium wilt resistance in protoplast-derived tomato plants.Theoretical and Applied Genetics 73: 164–169.

    Article  Google Scholar 

  • Stolle, K. & B. Schöber, 1984. Wirkung eines Toxins vonphytophthora infestans (Mont.) de Bary auf Kartoffelknollengewebe.Potato Research 27: 173–184.

    Article  CAS  Google Scholar 

  • Stolle, K. & B. Schöber, 1985. Wirkung eines Toxins vonPhytophthora infestans (Mont.) de Bary auf Kartoffelblätter.Potato Research 28: 389–402.

    CAS  Google Scholar 

  • Street, H. E., 1977. Cell (suspension) culture-techniques. In: H. E. Street (Ed.), Plant tissue and cell cultures. pp. 61–102. Blackwell Scientific Publications, Oxford.

    Google Scholar 

  • Toyoda, H. K., K. Shimizu, K. Chatani, N. Kita, Y. Matsuda & S. Ouchi, 1989. Selection of bacterial wilt-resistant tomato through tissue culture.Plant Cell Reports 8: 317–320.

    Google Scholar 

  • Umbeck, P. F. & B. G. Gengenbach, 1983. Reversion of male-sterile T-cytoplasm maize to male fertility in tissue culture.Crop Science 23: 584–588.

    CAS  Google Scholar 

  • Umaerus, V. & D. Lihnell, 1976. A laboratory method for measuring the degree of attack byPhytophthora infestans.Potato Research 19: 91–107.

    Google Scholar 

  • Vardi, A., E. Epstein & A. Breiman, 1986. Is thePhytophthora citrophthora culture filtrate a reliable tool for thein vitro selection of resistantCitrus variants?Theoretical and Applied Genetics 72: 569–574.

    Article  Google Scholar 

  • Wastie, R. L., 1991. Breeding for resistance. V. Methods of selecting and evaluating resistance. In: D. S. Ingram & P. H. Williams (Eds),Phytophthora infestans, the cause of late blight of potato. Advances in Plant Pathology, Vol. 7, pp. 210–220. Academic Press, London.

    Google Scholar 

  • Wenzel, G., 1985. Strategies in unconventional breeding for disease resistance.Annual Review of Phytopathology 23: 149–172.

    Article  Google Scholar 

  • Wenzel, G., S. C. Debnath, R. Schuchmann & B. Foroughi-Wehr, 1987. Combined application of classical and unconventional techniques in breeding for disease resistant potatoes. In: G. J. Jellis & D. E. Richardson (Eds), The production of new potato varieties: technological advances. pp. 277–288, Cambridge University Press.

  • Wenzel, G. & B. Foroughi-Wehr, 1990. Progeny tests of barley, wheat and potato regenerants from cell cultures afterin vitro selection for disease resistance.Theoretical and Applied Genetics 80: 359–365.

    Article  Google Scholar 

  • Witsenboer, H. M. A., C. E. van Schaik, R. J. Bino, H. J. M. Löffler, H. J. J. Nijkamp & J. Hille, 1988. Effects ofAlternaria f.sp.lycopersici toxins at different levels of tomato plant cell development.Plant Science 56: 253–260.

    Article  CAS  Google Scholar 

  • Wright, J. C. & M. L. Lacy, 1988. Increase of disease resistance in celery cultivars by regeneration of whole plants from cell suspension cultures.Plant Disease 72: 256–259.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Experimental Institute for Industrial Crops, Via Di Corticella 133, Bologna, Italy

    C. Cerato, L. M. Manici & S. Borgatti

  2. Department of Biology, University of Bologna, Via Belmeloro 8, Bologna, Italy

    R. Alicchio, R. Ghedini & A. Ghinelli

Authors
  1. C. Cerato
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. M. Manici
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Borgatti
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Alicchio
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Ghedini
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Ghinelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cerato, C., Manici, L.M., Borgatti, S. et al. Resistance to late blight (Phytophthora infestans (Mont.) de Bary) of potato plants regenerated fromin vitro selected calli. Potato Res 36, 341–351 (1993). https://doi.org/10.1007/BF02361801

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02361801

Additional keywords

Search

Navigation