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Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody

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Abstract

The expression of manganese superoxide dismutase (Mn−SOD) was studied immunohistochemically, using a specific monoclonal antibody, in surgically resected hepatocellular carcinoma (HCC) and noncancerous tissues from 47 patients (2 with well-differentiated HCC, 36 with moderately differentiated HCC, 8 with poorly differentiated HCC, and 1 with undifferentiated carcinoma). Cancer cells in 44 patients (93.6%) were positive for Mn−SOD. The staining pattern of cancer cells was mostly homogeneous in well-differentiated HCC, whereas it was heterogeneous in poorly differentiated HCC. Moreover, strongly positive immunoreactivity was observed in noncancerous liver tissues in all patients, especially in normal hepatocytes surrounding HCC, regenerative small hepatocytes in the tumor boundary, and mononuclear inflammatory cells in the necroinflammatory lesions. The positive immunoreactivity for Mn−SOD in patients with HCC appears to reflect increased production of the enzyme protein.

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References

  1. McCord JM, Keele BB, Fridovich I. An enzyme-based theory of obligate anaerobiosis: The physiological function of superoxide dismutase. Proc Natl Acad Sci USA 1971;68:1024–1027.

    CAS  PubMed  Google Scholar 

  2. McCord JM, Fridovich I. Superoxide dismutase—an enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244:6049–6055.

    CAS  PubMed  Google Scholar 

  3. Weisinger RA, Fridovich I. Mitochondrial superoxide dismutase: Site of synthesis and intramitochondrial localization. J Biol Chem 1973;248:4793–4796.

    Google Scholar 

  4. Marklund SL. Human copper-containing superoxide dismutase of high molecular weight. Proc Natl Acad Sci USA 1982;79: 7634–7638.

    CAS  PubMed  Google Scholar 

  5. Karlsson K, Marklund SL. Extracellular superoxide dismutase in the vascular system of mammals. Biochem J 1988;255:223–228.

    CAS  PubMed  Google Scholar 

  6. Oberley LW, Buettner GR. Role of superoxide dismutase in cancer: A review. Cancer Res 1979;39:1141–1149.

    CAS  PubMed  Google Scholar 

  7. Dovrat A, Gershon D. Rat lens superoxide dismutase and glucose-6-phosphate dehydrogenase: Studies on the catalytic activity and fate of enzyme antigen as a function of age. Exp Eye Res 1981;33:651–661.

    CAS  PubMed  Google Scholar 

  8. Iizuka S, Taniguchi N, Makita A. Enzyme-linked immunosorbent assay for human manganese-containing superoxide dismutase and its content in lung cancer. J Natl Cancer Inst 1984; 72:1043–1049.

    CAS  PubMed  Google Scholar 

  9. Kawaguchi T, Noji S, Uda T, et al. A monoclonal antibody against COOH-terminal peptide of human liver manganese superoxide dismutase. J Biol Chem 1989;264:5762–5767.

    CAS  PubMed  Google Scholar 

  10. Kawaguchi T, Suzuki K, Matsuda Y, et al. Serum manganese-superoxide dismutase: Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by an enzyme-linked immunosorbent assay using a monoclonal antibody. J Immunol Methods 1990;127: 249–254.

    Article  CAS  PubMed  Google Scholar 

  11. Ishikawa M, Yaginuma Y, Hayashi H, et al. Reactivity of a monoclonal antibody to manganese superoxide dismutase with human ovarian carcinoma. Cancer Res 1990;50:2538–2542.

    CAS  PubMed  Google Scholar 

  12. Gibson JB, Sobin LH. Histological typing of the tumours of the liver, biliary tract and pancreas (International histological classification of tumours. No. 20) Geneva: WHO, 1978.

    Google Scholar 

  13. Liver Cancer Study Group of Japan. The General Rules for the Clinical and Pathological Study of Primary Liver Cancer. 3rd ed. Tokyo: Kanehara Shuppan, 1992;29–76.

    Google Scholar 

  14. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29:577–580.

    CAS  PubMed  Google Scholar 

  15. Inagaki T, Katoh K, Takiya S, et al. Relationship between superoxide dismutase (SOD) and viral liver diseases. Gastroenterol Jpn 1992;27:382–389.

    CAS  PubMed  Google Scholar 

  16. Visner GA, Dougall WC, Wilson JM, et al. Regulation of manganese superoxide dismutase by lipopolysaccharide, interleukin-1, and tumor necrosis factor. J Biol Chem 1990;265:2856–2864.

    CAS  PubMed  Google Scholar 

  17. Wong GHW, Goeddel DV. Induction of manganese superoxide dismutase by tumor necrosis factor: Possible protective mechanism. Science 1988;242:941–944.

    CAS  PubMed  Google Scholar 

  18. Masuda A, Longo DL, Kobayashi Y, et al. Induction of mitochondrial manganese superoxide dismutase by interleukin 1. FASEB J 1988;2:3087–3091.

    CAS  PubMed  Google Scholar 

  19. Kawaguchi T, Takeyasu A, Matsunobu K, et al. Stimulation of Mn-superoxide dismutase expression by tumor necrosis factor-α: Quantitative determination of Mn−SOD protein levels in TNF-resistant and sensitive cells by ELISA. Biochem Biophys Res Commun 1990;171:1378–1386.

    Article  CAS  PubMed  Google Scholar 

  20. Wong GHW, Elwell JH, Oberley LW, et al. Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor. Cell 1989;58:923–931.

    Article  CAS  PubMed  Google Scholar 

  21. Asoh K, Watanabe Y, Mizoguchi H, et al. Induction of manganese superoxide dismutase by tumor necrosis factor in human breast cancer MCF-7 cell line and its TNF-resistant variant. Biochem Biophys Res Commun 1989;162:794–801.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Second Department of Pathology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, 216, Kawasaki, Kanagawa, Japan

    Yoshi Aida, Shiro Maeyama, Toshifumi Takakuwa & Toshiyuki Uchikoshi

  2. Sanraku Hospital, 2-5 Kanda-Surugadai, Chiyoda-ku, 101, Tokyo, Japan

    Yasuo Endo

  3. Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, 565, Suita, Osaka, Japan

    Keiichiro Suzuki & Naoyuki Taniguchi

Authors
  1. Yoshi Aida
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  2. Shiro Maeyama
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  3. Toshifumi Takakuwa
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  4. Toshiyuki Uchikoshi
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  5. Yasuo Endo
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  6. Keiichiro Suzuki
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  7. Naoyuki Taniguchi
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Aida, Y., Maeyama, S., Takakuwa, T. et al. Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody. J Gastroenterol 29, 443–449 (1994). https://doi.org/10.1007/BF02361241

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  • DOI: https://doi.org/10.1007/BF02361241

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