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Analysis of potato microtuber proteins by sodium dodecylsulfate polyacrylamide gel electrophoresis

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Summary

Protein banding patterns of 119 lines (85 Japanese potato lines and introductions, 22 dihaploids, 1 wild source (Solanum bulbocastanum Dun.), 10 transformants and 1 somaclonal variant) were compared by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Microtubers formed in vitro were used as the source of plant materials. There were more than 15 bands in the 35–20 kDa molecular weight range and less than 10 bands in the 65–40 kDa range. The bands in the 65–40 kDa range which corresponded to the potato tuber storage protein patatin were focussed to about 40 kDa after heat treatment. The results indicate a distinct difference in the banding patterns among potato lines.

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Rajapakse, D.P., Imai, T. & Ishige, T. Analysis of potato microtuber proteins by sodium dodecylsulfate polyacrylamide gel electrophoresis. Potato Res 34, 285–293 (1991). https://doi.org/10.1007/BF02360501

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