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An immunohistochemical employer monoclonal antibodies against Lea, sialyl Lea, Lex, and sialyl Lex antigens in primary colorectal, carcinomas and lymph node and hepatic lesions

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Abstract

The immunohistochemical expression of sialylated and non-sialylated forms of both Lex and Lea were studied in 87 carcinomas and 42 normal mucosal specimens of colon and rectum, as well as in 32 metastatic lymph nodes and 9 hepatic lesions, using an indirect immunoperoxidase staining. Their antigens were expressed in normal mucosa with the following frequencies: Lea, 95.2% (40/42); sialyl Lea, 88.1% (37/42); Lex, 95.2% (40/42); and sialyl Lex, 17.0% (7/42), whereas in carcinomas, the respective rate of frequency were: 78.2% (68/87); 78.2% (68/87); 90.8% (79/87); and 93.1% (81/87). Sialyl Lex antigen showed the highest tumor specificity compared to other antigens. In three normal mucosal specimens and four carcinomas with Le(a−b−) phenotype, the expression of type 1 antigens (Lea and sialyl Lea) was not consistent, whereas type 2 antigens (Lex and sialyl Lex) were consistently observed in carcinomas. The staining of type 1 antigens and Lex was decreased in metastatic lesions compared with primary carcinomas, whereas sialyl Lex antigen had the same positive-staining rate in both. Metastatic carcinoma expressed the sialylated form more predominantly than the non-sialylated form in type 2 antigens whereas the opposite result was observed in type 1 antigens. These results suggested that: (a) sialyl Lex, defined by monoclonal antibody CSLEX1, may be useful as a tumor-associated antigen in colorectal carcinoma, and (b) the alteration of Lewis-related carbohydrate antigens in cancer cell membranes, including sialylation and/or aberrant glycosylation, may be related to metastatic behavior.

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Authors and Affiliations

  1. First Department of Surgery, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852, Nagasaki, Japan

    Tohru Nakagoe, Hiroyuki Kusano, Hiroyoshi Ayabe & Masao Tomita

  2. Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852, Nagasaki, Japan

    Kiyoyasu Fukushima & Masaki Hirota

  3. Division of Blood Transfusion, Nagasaki University Hospital, 1-7-1 Sakamoto, 852, Nagasaki, Japan

    Shimeru Kamihira

Authors
  1. Tohru Nakagoe
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  2. Kiyoyasu Fukushima
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  3. Masaki Hirota
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  4. Hiroyuki Kusano
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  5. Hiroyoshi Ayabe
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  6. Masao Tomita
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  7. Shimeru Kamihira
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Nakagoe, T., Fukushima, K., Hirota, M. et al. An immunohistochemical employer monoclonal antibodies against Lea, sialyl Lea, Lex, and sialyl Lex antigens in primary colorectal, carcinomas and lymph node and hepatic lesions. J Gastroenterol 29, 129–138 (1994). https://doi.org/10.1007/BF02358673

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  • DOI: https://doi.org/10.1007/BF02358673

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