Summary
Uridine-H3 was incorporated into meristem tips of both healthy and PLRV-infected potato plants, of the cultivars, Majestic and Primura. Autoradiograms of tips pretreated with actinomycin D showed that 13 of 14 and 11 of 14 respectively, were virus free in the dome and first 4 leaf primordia, 1 and 3 were infected in the 4th leaf primordium, and 1 of Primura also in its 3rd primordium.
The highest plantlet yield from cultures in vitro of meristem tips that included 4 leaf primordia (7 to 9 plantlets per meristem), was obtained by growing them in sequence on 3 sequential media, each based on Murashige and Skoog basic medium complemented with various hormones. The 2nd medium, containing benzylaminopurine (0.5 mg/l) and gibberellic acid (0.5 mg/l), elicited callus and the subsequent formation of adventitious buds. Of the plantlets of the cvs Vivaks, Primura and Majestic, 88, 91 and 100 %, respectively, were PLRV-free.
Propagation in vitro, by the single-node cutting technique and tuberlet production, were successful.
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Faccioli, G., Rubies-Autonell, C. & Resca, R. Potato leafroll virus distribution in potato meristem tips and production of virus-free plants. Potato Res 31, 511–520 (1988). https://doi.org/10.1007/BF02357889
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DOI: https://doi.org/10.1007/BF02357889