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Transposable elements behavior following viral genomic stress inDrosophila melanogaster inbred line

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Abstract

To analyze the behavior of endogenous transposable elements under genomic stress, aDrosophila melanogaster inbred line was submitted to three kinds of viral perturbations. First, a retroviral plasmid containing the avian Rous Associated Virus type 2 (RAV-2) previously deleted for the viral envelope coding gene (env) was introduced by P element transformation into theDrosophila genome. An insertion of this avian retroviral sequence was detected byin situ hybridization in site 53C on polytene chromosome arm 2R. Second,Drosophila embryos were injected with RAV-2 particles produced by cell culture after transfection with the retroviral plasmid. Third, theDrosophila melanogaster inbred line was stably infected by the sigma native virus. It appears that neither the offspring of the flies in which the viral DNA was found integrated nor those from the infected sigma flies showed copia or mdgl element mobilization. Injection of the avian RAV-2 particles led, however, to the observation of somatic transpositions of mdgl element on the 2L chromosome, the copia element insertion pattern remaining stable. Thus, endogenous transposable elements show more instability in sublines injected with exogenous viral particles than in a transgenic subline containing a foreign viral insert, all transposable elements not being equally sensitive to such genomic stress.

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Authors and Affiliations

  1. Laboratoire de Biométrie, Génétique et Biologic des Populations, Université Claude Bernard, UMR 5558, Lyon 1, 69622, Villeurbanne Cedex, France

    Isabelle Jouan-Dufournel & Christian Biémont

  2. Centre de Génétique Moléculaire et Cellulaire, Université Claude Bernard, UMR 106, Lyon 1, 69622, Villeurbanne Cedex, France

    François-Loïc Cosset & Gérard Verdier

  3. Centre de Genetique Moleculaire, UPR 2420, CNRS, 91198, Gif-sur-Yvette, France

    Didier Contamine

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  1. Isabelle Jouan-Dufournel
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  2. François-Loïc Cosset
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  3. Didier Contamine
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  4. Gérard Verdier
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  5. Christian Biémont
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Correspondence to: I. Jouan-Dufournel

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Jouan-Dufournel, I., Cosset, FL., Contamine, D. et al. Transposable elements behavior following viral genomic stress inDrosophila melanogaster inbred line. J Mol Evol 43, 19–27 (1996). https://doi.org/10.1007/BF02352295

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  • DOI: https://doi.org/10.1007/BF02352295

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