Skip to main content
SpringerLink
Log in

Effects of 16, 16-dimethyl prostaglandin E2 on lysosomal membrane stability in rat stomach

  • Alimentary Tract
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

The lysosomal membrane encloses numerous hydrolytic enzymes and prevents the cytoplasm from being damaged by these enzymes. It is possible that the fragility of this membrane may be implicated in the pathogenesis of gastric mucosal damage. We investigated the effects of 16,16-dimethyl prostaglandin E2 (dmPGE2), which is known to protect the gastric mucosa from various noxious agents, on lysosomal membrane stability in the rat stomach. Sodium taurocholate (TC) was used as the damaging agent. To assess lysosomal membrane stability in the gastric mucosa, we assayed acid phosphatase released from lysosomes isolated from a gastric mucosal homogenate. To assess lysosomal membrane stability in gastric surface epithelial cells, we used laser scanning confocal microscopy to observe the fading of red fluorescence in living cells vitally stained with acridine orange. Exogenous dmPGE2 enhanced lysosomal membrane stability in the gastric mucosa, whereas TC decreased it. In gastric surface epithelial cells, exogenous dmPGE2 protected the cells against TC-induced damage and prevented TC-induced decreased lysosmal membrane stability. It was concluded that a decrease in lysosomal membrane stability seemed to be closely involved in the pathogenesis of gastric mucosal damage. Moreover, it appears that stabilization of the lysosomal membrane by exogenous dmPGE2 may contribute to its protective effect in the gastric mucosa, both at the level of gastric surface epithelial cells and in regard to the entire gastric mucosa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. de Duve C. The lysosome. Sci Am 1963;208:64–72.

    Google Scholar 

  2. Kornfeld S, Mellman I. The biogenesis of lysosomes. Annu Rev Cell Biol 1989;5:483–525.

    Article  CAS  PubMed  Google Scholar 

  3. Ferguson WW, Edmonds AW, Starling JR, Wangensteen SL. Protective effect of prostaglandin E1 (PGE1) on lysosomal enzyme release in serotonin-induced gastric ulceration. Ann Surg 1973;177:648–654.

    CAS  PubMed  Google Scholar 

  4. Waldron-Edward D, Boutros MIR, Himal HS. Effect of bile on lysosomal stability in the mucosa of the canine gastric antrum. Gastroenterology 1977;73:980–984.

    CAS  PubMed  Google Scholar 

  5. Himal HS, Mowat C. Prostaglandin cytoprotection and lysosomal stability in acute canine gastric erosions. Can J Sur 1983; 26:142–143.

    CAS  Google Scholar 

  6. Robert A, Nezamis JE, Lancaster C, Hanchar AJ. Cytoprotection by prostaglandins in rats. Gastroenterology 1979;77:433–443.

    CAS  PubMed  Google Scholar 

  7. Miller TA, Jacobson ED. Gastrointestinal cytoprotection by prostaglandins. Gut 1979;20:75–87.

    CAS  PubMed  Google Scholar 

  8. Lacy ER, Ito S. Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with a prostaglandin. Gastroenterology 1982;83:619–625.

    CAS  PubMed  Google Scholar 

  9. Terano A, Ota S, Mach T, et al. Prostaglandin protects against taurocholate-induced damage to rat gastric mucosal cell culture. Gastroenterology 1987;92:669–677.

    CAS  PubMed  Google Scholar 

  10. Ignarro LJ. Effects of anti-inflammatory drugs on the stability of rat liver lysosomes in vitro. Biochem Pharmacol 1971;20:2847–2860.

    CAS  PubMed  Google Scholar 

  11. Torriani A. Influence of inorganic phosphate in the formation of phosphatases byEscherichia coli. Biochim Biophys Acta 1960; 38:460–469.

    Article  CAS  PubMed  Google Scholar 

  12. Saitoh O, Maemura K, Ohshiba S, Fukuda M. Role of lysosomal membrane glycoproteins in defense mechanisms of human colon cancer cells. Cytoprotect Cytobiol 1992;10:225–230.

    Google Scholar 

  13. Matuoka K, Tanaka M, Mitsui Y, Murota S. Cultured rabbit gastric epithelial cells producing prostaglandin I2. Gastroenterology 1983;84:498–505.

    CAS  PubMed  Google Scholar 

  14. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. J Immunol Methods 1983[65:55–63.

    Article  CAS  PubMed  Google Scholar 

  15. Ota S, Tsukahara H, Terano A, et al. Protective effect of tauroursodeoxycholate against chenodeoxycholate-induced damage to cultured rabbit gastric cells. Dig Dis Sci 1991;36: 409–416.

    Article  CAS  PubMed  Google Scholar 

  16. Allison AC, Young MR. Uptake of dyes and drugs by living cells in culture. Life Sci 1964;3:1407–1414.

    CAS  PubMed  Google Scholar 

  17. Rundquist I, Olsson M, Brunk U. Cytofluorometric quantitation of acridine orange uptake by cultured cells. Acta Pathol Microbiol Scand (A) 1984;92:303–309.

    CAS  Google Scholar 

  18. Cure IN. Inereased resistance of the gastric mucosal barrier to barrier breakers in the rat. Gastroenterology 1979;77:309–312.

    Google Scholar 

  19. Angerson WJ, Geraghty JG, Carter DC. Taurocholate-induced gastric mucosal injuries in experimental portal hypertension. Gut 1992;33:170–174.

    CAS  PubMed  Google Scholar 

  20. Soll AH, Whittle BJR. Interaction between prostaglandins and cyclic AMP in the gastric mucosa. Prostaglandin 1981;21[Suppl]: 39–45.

    CAS  Google Scholar 

  21. Konturek SJ, Brzozowski T, Radecki T, Dobrzanska M. Generation of endogenous prostaglandins and thromboxanes in taurocholate-induced gastric mucosal lesion. Scand J Gastroenterol 1984;19:91–96.

    CAS  Google Scholar 

  22. Mutoh H, Hiraishi H, Ota S, et al. Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells. Am J Physiol 1990;258:G603-G609.

    CAS  PubMed  Google Scholar 

  23. Mutoh H, Hiraishi H, Ota S, et al. Protective role of intracellular glutathione against ethanol-induced damage in cultured rat gastric mucosal cells. Gastroenterology 1990;98:1452–1459.

    CAS  PubMed  Google Scholar 

  24. Mutoh H, Ota S, Hiraishi H, et al. Reduced glutathione protects cultured gastric mucosal cells from suckling rats against acid. Am J Physiol 1991;261:G65-G70.

    CAS  PubMed  Google Scholar 

  25. Arakawa T, Fukuda T, Nakamura A, et al. Protection by 16,16-dimethyl PGE2 of surface epithelial, parietal, and chief cells isolated from rat stomach (in Japanese with English abstract). Jpn J Gastroenterol 1989;86:1031–1036.

    CAS  Google Scholar 

  26. Konda Y, Nishisaki H, Nakano O, et al. Prostaglandin protects isolated guinea pig chief cells against ethanol injury via an increase in diacylglycerol. J Clin Invest 1990;86:1897–1903.

    CAS  PubMed  Google Scholar 

  27. Fantone JC, Marasco WA, Elgas, LJ, Ward PA. Stimulus specificity of prostaglandin inhibition of rabbit polymorphonuclear leukocyte lysosomal enzyme release and superoxide anion production. Am J Pathol 1984;115:9–16.

    CAS  PubMed  Google Scholar 

  28. Hogaboam CM, Bissonnette EY, Chin BC, et al. Prostaglandins inhibit inflammatory mediator release from rat mast cells. Gastroenterology 1993;104:122–129.

    CAS  PubMed  Google Scholar 

  29. Takamatsu T, Fujita S. Confocal laser scanning microscopy for image cytometry (in Japanese with English abstract). Nippon Rinsho (Jpn J Clin Med) 1992;50:2524–2546.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Second Department of Internal Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, 569, Osaka, Japan

    Osamu Saitoh, Ken Nakagawa, Shuji Asada, Kazunori Sugi, Ichiro Hirata & Saburo Ohshiba

Authors
  1. Osamu Saitoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ken Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuji Asada
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazunori Sugi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ichiro Hirata
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Saburo Ohshiba
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saitoh, O., Nakagawa, K., Asada, S. et al. Effects of 16, 16-dimethyl prostaglandin E2 on lysosomal membrane stability in rat stomach. J Gastroenterol 29, 703–709 (1994). https://doi.org/10.1007/BF02349274

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02349274

Key words

Search

Navigation