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Field measurements of nitrogen-fixing activity of intact saplings ofAlnus maximowiczii in the subalpine zone of Mt Fuji

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Ecological Research

Abstract

Nitrogen-fixing (acetylene-reducing) activity of intact saplings ofAlnus maximowiczii was measured under natural conditions in the subalpine zone of Mt Fuji. The nitrogen-fixing activity was detected from the middle of June when expansion of leaves had just begun to the end of October when the shedding of leaves was almost completed. Diurnal changes in the activity were almost parallel with those of ground temperature. The measured nitrogen-fixing activity was related to ground temperature and total leaf area. Using this relation, annual nitrogen fixation was estimated from the data of ground temperature and leaf area measured in the field. The amount of annual nitrogen fixation was almost the same as that of nitrogen used for annual growth. It was concluded that nitrogen fixation by nodules made a considerable contribution to the nitrogen economy in the saplings ofA. maximowiczii.

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References

  • Akkermans A. D. L. &van Dijk C. (1976) The formation and nitrogen-fixing activity of the root nodules ofAlnus glutinosa under field conditions. In:Symbiotic Nitrogen Fixation in Plants IBP 7 (ed. P. S. Nunman), pp. 511–20. Cambridge University Press, Cambridge.

    Google Scholar 

  • Bormann B. T. &Sidle R. C. (1990) Changes in productivity and distribution of nurrients in a chronosequence at Glacier Bay National Park, Alaska.J. Ecol. 78: 561–78.

    Google Scholar 

  • Chapin F. S., III &Kedrowski R. A. (1983) Seasonal changes in nitrogen and phosphorus fractions and autumn retranslocation in evergreen and deciduous taiga trees.Ecology 64: 376–91.

    CAS  Google Scholar 

  • Chapin F. S., III &Moilanen L. (1991) Nutritional controls over nitrogen and phosphorus resorption from Alaskan birch leaves.Ecology 72: 709–15.

    Google Scholar 

  • Fujimura Y. & Sakagami Y. (1984) Seasonal changes of growth in the saplings of threeBetula species (1) Elongation, thickening growth and characters.Trans. 33rd. Annu. Meet. Hokkaido Branch. Jpn. For. Soc., 39–41 (in Japanese).

  • Hardy R. W. F., Burns R. C. &Holsten R. D. (1973) Applications of the acetylene-ethylene assay for measurement of nitrogen fixation.Soil Biol. Biochem. 5: 47–81.

    Article  CAS  Google Scholar 

  • Hardy R. W. F., Holsten R. D., Jackson E. K. &Burns R. C. (1968) The acetylene-ethylene assay for N2 fixation: Laboratory and field evaluation.Plant Physiol. 43: 1185–1207.

    CAS  Google Scholar 

  • Huss-Danell K. (1978) Nitrogenase activity measurements in intact plants ofAlnus incana.Physiol. Plant. 43: 372–6.

    CAS  Google Scholar 

  • Huss-Daneli K. &Sellstedt A. (1983) Nitrogenase activity in response to restricted shoot growth inAlnus incana.Can. J. Bot. 61: 2949–55.

    Google Scholar 

  • Huss-Danell K. &Sellstedt A. (1985) Nitrogenase activity in response to darkening and defoliation ofAlnus incana.J. Exp. Bot. 36: 1352–8.

    CAS  Google Scholar 

  • Johnsrud S. C. (1978) Nitrogen fixation by root nodules ofAlnus incana in a Norwegian forest ecosystem.Oikos 30: 475–9.

    Google Scholar 

  • Maruta E. (1980) Plant establishment in unproductive habirats as related to dry matter production and water relations. PhD thesis, University of Tokyo.

  • Masuzawa T. (1985) Ecological studies on the timberline of Mt Fuji. I. Structure of plant community and soil development on the timber line.Bot. Mag. Tokyo 98: 15–28.

    Google Scholar 

  • Sakio H. &Masuzawa T. (1987) Ecological studies on the timber line of Mt Fuji. II. Primary productivity ofAlnus maximowiczii dwarf forest.Bot. Mag. Tokyo 100: 349–63.

    Google Scholar 

  • Sakio H. &Masuzawa T. (1992) Ecological studies on the timber line of Mt Fuji. III. Seasonal changes in nitrogen content in leaves of woody plants.Bot. Mag. Tokyo 105: 47–52.

    CAS  Google Scholar 

  • Sharma E. &Ambasht R. S. (1984) Seasonal variation in nitrogen fixation by different ages of root nodules ofAlnus nepalensis plantations, in the eastern Himalayas.J. Appl. Ecol. 21: 265–70.

    CAS  Google Scholar 

  • Sprent J. I. &Sprent P. (1990)Nitrogen fixing organisms. Pure and applied aspects. Chapman and Hall, London.

    Google Scholar 

  • Stewart W. D. P., Fitzgerald G. P. &Burris R. H. (1967)In situ studies on N2 fixation using the acetylene reduction technique.Proc. Natl Acad. Sci. USA 58: 2071–8.

    CAS  PubMed  Google Scholar 

  • Sundström K-R. &Huss-Danell K. (1987) Effects of water stress on nitrogenase activity inAlnus incana.Physiol. Plantarum 70: 342–8.

    Google Scholar 

  • Tagawa H. (1964) A study of the volcanic vegetation in Sakura-jima, south-west Japan I. Dynamics of vegetation.Mem. Fac. Sci. Kyushu Univ., Ser. E (Biol.)3: 165–228.

    Google Scholar 

  • Tezuka Y. (1961) Development of vegetation in relation to soil formation in the volcanic island of Oshima, Izu, Japan,Jpn. J. Bot 17: 371–402.

    Google Scholar 

  • Vikman P.-Å., Lundquist P-O. &Huss-Danell K. (1990) Respiratory capacity, nitrogenase activity and structural changes ofFrankia, in symbiosis withAlnus incana, in response to prolonged darkness.Planta 182: 617–25.

    Article  CAS  Google Scholar 

  • Virtanen A. I. (1957) Investigation on nitrogen fixation by the alder. II. Associated culture of spruce and inoculated alder without combined nitrogen.Physiol. Plantarum 10: 164–9.

    CAS  Google Scholar 

  • Vitousek P. M. &Walker I. R. (1989) Biological invasion byMyrica faya in Hawai'i: Plant demography, nitrogen fixation, ecosystem effects.Ecol. Monogr. 59: 247–65.

    Google Scholar 

  • Wheeler C. T. (1971) The causation of the diurnal changes in nitrogen fixation in the nodules ofAlnus glutinosa.New Phytol. 70: 487–95.

    Google Scholar 

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Tsutsumi, H., Nakatsubo, T. & Ino, Y. Field measurements of nitrogen-fixing activity of intact saplings ofAlnus maximowiczii in the subalpine zone of Mt Fuji. Ecol. Res. 8, 85–92 (1993). https://doi.org/10.1007/BF02348611

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