Abstract
Protoplasts were isolated from the leaves of sterile plants ofPopulus euphratica Oliv. by using 1% Cellulase “Onozuka” RS and 0.25% Pectolyase Y-23 in 0.6m of mannitol solution. Protoplasts were cultured in modified Murashige and Skoog's (MS) medium which contained no ammonium ions but was supplemented with BAP (6-benzylaminopurine), 2,4-D (2,4- dichlorophenoxy-acetic acid), and 1% sucrose at the cell density of 9×104/ml. Cell divisions occurred in every culture medium, especially in the medium containing 0.5 mg/l of BAP and 0.1 mg/l of 2,4-D, in which callus was successfully induced by successive culture through cell cluster formation. Shoots were regenerated from the callus, and their growth was enhanced on 1/2 MS medium containing 0.8 mg/l of BAP. Finally, shoots were rooted and plantlets were regenerated on 1/2 MS medium without a hormone.
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Literature cited
Durzan, D.J. (1982) Cell and tissue culture in forest industry.In Tissue culture in forestry. Bonga, J.M. and Durzan, D.J. (eds.), 420pp, Martinus Nijhoff Pub., Dordrecht, 36–71.
FAO (1979) Poplars and willows in wood production and land use. FAO Forestry series. 328pp, FAO, Rome, 13–45.
Ito, K., Doi, K., and Shibata, M. (1987) Regeneration of plants from protoplasts of poplar shoot primordia. Jpn. J. Breed 37 (Suppl. 2): 4–5. (in Japanese)
Kang, J.-M., Kojima., K., and Ide, Y. (1992) Establishment of tissue culture system of Chinese poplars:Populus tomentosa andPopulus alba cv. Pyramidalis XPopulus tomentosa. Bull. Tokyo Univ. Forests 88: 127–133.
Lee, J.-S., Lee, S.-K., Jang, S.-S., and Lee, J.-J. (1987) Plantlet regeneration from callus protoplasts ofPopulus nigra. Res. Rep. Inst. For. Gen. Korea 23: 143–148 (in Korean)
Mohri, T. and Miura, K. (1992) Plantlet regeneration from leaf protoplast ofPopulus nigra L. var.italica Koehne. Bull. Hokkaido Univ. For. 49: 261–275. (in Japanese with English summary)
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Park, Y.-K. and Son, S.-H. (1988) Culture and regeneration ofPopulus alba X glandulosa leaf protoplasts isolated fromin vitro cultured explant. Jour. Korean For. Soc. 77: 208–215. (in Korean)
Russell, J.A. and McCown, B.H. (1986) Culture and regeneration ofPopulus leaf protoplasts isolated from non-seedling tissue. Plant Sci. 46: 133–142.
Russell, J.A. and McCown, B.H. (1988) Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones. Plant Cell Rep. 7: 59–62.
Saito, A., Hosoi, Y., Ishii, K., and Sato, T. (1987) Callus formation from protoplasts of mesophyll cells ofPopulus plantlets. J. Jpn. For. Soc. 69: 472–477.
Sasamoto, H., Hosoi, Y., Ishii, K., Sato, T., and Saito, A. (1989a) Factors affecting the formation of callus from leaf protoplasts ofPopulus alba. J. Jpn. For. Soc. 71: 449–455.
Sasamoto, H., Hosoi, Y., Ishii, K., Sato, T., and Saito, A. (1989b) Callus formation and plantlet regeneration from mesophyll protoplasts ofPopulus sieboldii. Trans. 100th Mtg. Jpn. Soc.: 531–532. (in Japanese)
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Kang, JM., Kojima, K., Ide, Y. et al. Plantlet regeneration from leaf protoplasts ofPopulus euphratica . J. For. Res. 1, 99–102 (1996). https://doi.org/10.1007/BF02348311
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DOI: https://doi.org/10.1007/BF02348311