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The role of wall calcium in the extension of cell walls of soybean hypocotyls

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Abstract

Calcium crosslinks are load-bearing bonds in soybean (Glycine max (L.) Merr.) hypocotyl cell walls, but they are not the same load-bearing bonds that are broken during acid-mediated cell elongation. This conclusion is reached by studying the relationship between wall calcium, pH and the facilitated creep of frozenthawed soybean hypocotyl sections. Supporting data include the following observations: 1) 2-[(2-bis-[carboxy-methyl]amino-5-methylphenoxy)methyl]-6-methoxy-8-bis[carboxymethyl]aminoquinoline (Quin 2) and ethylene glycol-bis(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) caused only limited facilitated creep as compared with acid, despite removal of comparable or larger amounts of wall calcium; 2) the pH-response curves for calcium removal and acid-facilitated creep were different; 3) reversible acid-extension occurred even after removal of almost all wall calcium with Quin 2; and 4) growth of abraded sections did not involve a proportional loss of wall calcium. Removal of wall calcium, however, increased the capacity of the walls to undergo acid-facilitated creep. These data indicate that breakage of calcium crosslinks is not a major mechanism of cell-wall loosening in soybean hypocotyl tissues.

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Abbreviations

EGTA:

ethylene glycol-bis(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

ExE:

exponential phase of facilitated creep

LBBs:

load-bearing bonds

LE:

linear phase of facilitated creep

Quin 2:

2-[(2-bis-[carboxymethyl]-amino-5-methylphenoxy)methyl] -6-methoxy-8-bis[carboxymethyl]aminoquinoline

PEx:

Instron plastic extensibility

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Authors and Affiliations

  1. Department of Botany, KB-15, University of Washington, 98195, Seattle, WA, USA

    Sarbjit S. Virk & Robert E. Cleland

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  1. Sarbjit S. Virk
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  2. Robert E. Cleland
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Additional information

This research was supported by Department of Energy grant DE-FG06-88ER13830 and NASA grant NAGW 1394. The authors are grateful to Dr. David Rayle (San Diego State University, Cal.) for stimulating discussions and comments during the course of this work.

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Virk, S.S., Cleland, R.E. The role of wall calcium in the extension of cell walls of soybean hypocotyls. Planta 182, 559–564 (1990). https://doi.org/10.1007/BF02341032

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