Abstract
Age-related deposits within the inner and outer collagenous zones (ICZ/OCZ) of Bruch's membrane have been studied in normal aged human eyes. Electron microscopic examination of tissue from the macular region of 28 post-mortem eyes has shown that age-related deposits comprise mainly coated membrane-bound (CMB) bodies and their fragments. These bodies appear to be released from the RPE via the basal plasma membrane. Morphometric analysis of the average thickness and distribution of age-related deposits has demonstrated that as the thickness of the deposit increases with age, the major part is contained within the OCZ. The ICZ shows only a minimal increase in thickness during life. These results suggest that in the normal ageing eye the inner layers of Bruch's membrane (RPE basement membrane, ICZ and elastic layer) permit an unimpeded flow of CMB bodies from the RPE through to the OCZ.
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References
Altstiel L, Branton D (1983) Fusion of coated vesicles with lysosomes: measurement with a fluorescence assay. Cell 32:921–929
Brown MS, Anderson RGW, Goldstein JL (1983) Recycling receptors: the round-trip itinerary of migrant membrane proteins. Cell 32:663–667
Feeney-Burns L, Ellersieck MR (1985) Age-related changes in the ultrastructure of Bruch's membrane. Am J Ophthalmol 100:686–697
Friend DS, Farquhar MG (1967) Functions of coated vesicles during protein absorption in the rat vas deferens. J Cell Biol 35:357–376
Herman KC, Steinberg RH (1982a) Phagosome movement and the diurnal pattern of phagocytosis in the tapetal retinal pigment epithelium of the opossum. Invest Ophthalmol Vis Sci 23:277–290
Herman KC, Steinberg RH (1982b) Phagosome degradation in the tapetal retinal pigment epithelium of the opossum. Invest Ophthalmol Vis Sci 23:291–304
Hogan MJ (1972) Role of the retinal pigment epithelium in macular disease. Am Acad Ophthalmol Otolaryn Trans 76:64–80
Hogan MJ, Alvarado J (1967) Studies on the human macula. IV. Aging changes in Bruch's membrane. Arch Ophthalmol 77:410–420
Hogan MJ, Alvarado JA, Weddell JE (1971) Choroid. In: Histology of the human eye. Saunders, Philadelphia, pp 320–392
Ishibashi T, Sorgente N, Patterson R, Ryan SJ (1986) Aging changes in Bruch's membrane of monkeys: an electron microscopic study. Ophthalmologica 192:179–190
Nakaizumi Y, Hogan MJ, Feeney L (1964) The ultrastructure of Bruch's membrane. III. The macular area of the human eye. Arch Ophthalmol 72:395–400
Ockleford CD (1981) Coated vesicles. In: Harris JR (ed) Electron microscopy of proteins, vol 1. Academic Press, London, pp 255–296
Orzalesi N, Fossarello M, Carta S, Del Fiacco G, Diaz G (1982) Identification and distribution of coated vesicles in the retinal pigment epihelium of man and rabbit. Invest Ophthalmol Vis Sci 23:689–696
Sarks SH (1976) Ageing and degenerating in the macular region: a clinico-pathological study. Br J Ophthalmol 60:324–341
Spitznas M, Hogan MJ (1970) Outer segments of photoreceptors and the retinal pigment epithelium. Arch Ophthalmol 84:810–819
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Killingsworth, M.C. Age-related components of Bruch's membrane in the human eye. Graefe’s Arch Clin Exp Ophthalmol 225, 406–412 (1987). https://doi.org/10.1007/BF02334166
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DOI: https://doi.org/10.1007/BF02334166