Summary
Sepabeads FP series with a variety of anion-exhanger type functional groups have been examined as largescale chromatographic separation media for proteins. The adsorption-desorption behavior of proteins has been found to be dependent mainly on the type and partly on the content of the attached amino ligands. The effect of the attached groups are discussed using batch measurement data. It has been found that the existence of ethanol residue on the nitrogen atom incrases the hydrophilic nature of quaternary ammonium type packings and thus gives a higher protein adsorption capacity. Among weakly basic type anionexchangers, polyethyleneimine-modified packings showed very large protein capacities.
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References
There are a number of published works on chromatographic supports for biopolymer separation. For example:O. Mikes et al., J. Chromatogr.,119, 339 (1976).
W. J. Jankowski et al., Biochem.15, 5182 (1976).
G. V. Samsonov, “Ion-exchange sorption and preparative chromatography of biologically active molecules”, Plenum, 1986.
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H. Kusano et al., Prepr. p. 38. 5th Symposium on Ion Exchange, Hungary, 1986. See also; Technical Data Sheets of Sepabeads FP series, available from Mitsubishi Chemical Industries Limited. Marunouchi, Chiyoda-ku, Tokyo, Japan.
H. Kusano et al., Reactive Polymers, in press.
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Presented at HPLC '87, Amsterdam, 1987.
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Takayanagi, H., Ito, T., Kubo, Y. et al. Sepabeads FP series: New packing materials for industrial-scale separation of biopolymers. Chromatographia 25, 647–651 (1988). https://doi.org/10.1007/BF02327665
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DOI: https://doi.org/10.1007/BF02327665