Summary
A collaborative study was organised, according to the ISO 5725 protocol, for the evaluation of methods of residue analysis of chloramphenicol on a national basis and as potential reference methods for the European Community. The methods to be examined were RIA and HPLC.
Reference samples were prepared with defined contents of chloramphenicol in the order of 1 μg/kg in milk and 10 μg/kg in meat, respectively. The analytical methods used for the determination of the “true” value of chloramphenicol contents were radioimmunoassay (RIA) and gas chromatography with either electron capture detection (GC/ECD) or mass specific detection (GC/MSD). Each concentration level was analysed 16 times by RIA, 5 times by GC/ECD and twice by GC/MSD.
An additional object of our studies was the examination of the packed and coded samples for homogeneity. The influence of duration and temperature of storage and the influence of irradiation were investigated.
Similar content being viewed by others
Abbreviations
- CAP:
-
Chloramphenicol
- RIA:
-
Radioimmunoassay
- GC/ECD:
-
Gas Chromatography with Electron Capture Detector
- GC/MSD:
-
Gas Chromatography with Mass Specific Detector
- NCl:
-
Negative Chemical lonisation
- El:
-
Electron Impact
- CV:
-
Coefficient of Variation
- n:
-
number of replicates
- \(\bar x\) :
-
mean value
References
A. A. Yunis, G. R. Blomberg, Chloramphenicol toxicity: Clinical features and pathogenesis, Progr. Hemat.,4, 138–159 (1964).
D. Arnold, A. Somogyi, J. of Association of Official Analytical Chemists68, 984–990 (1985).
A. Boertz, Radioimmunologische und Gaschromatographische Untersuchungen zum Rückstandsverhalten von Chloramphenicol beim Schwein, Dissertation, Technische Universität Berlin FB 13 (1984).
A. K. Boertz, D. Arnold, A. Somogyi, Z. Ernährungswiss.24, 113–119 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Balizs, G., Arnold, D. Reference standards for residue analysis of chloramphenicol in meat and milk: a critical study. Chromatographia 27, 489–493 (1989). https://doi.org/10.1007/BF02319571
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02319571