Abstract
Nodal stem segments ofDioscorea bulbifera were induced to form plantlets in vitro. Rooted plantlets were obtained on Murashige-Skoog [14] revised medium supplemented initially with 5 mg 1−1 kinetin and subsequently with 5 mg l−1 indole-butyric acid. By increasing the kinetin concentration from 5 mg l−1 to 10 mg l−1, the number of shoots forming per node was increased from five to eight. When kinetin was substituted with 6-benzylaminopurine at only 1 mg l−1, nine shoots formed on each node. Each shoot could be excised from the node and induced to form a new crop of multiple shoots. Rooted plantlets could be successfully transferred to in vivo conditions.
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Forsyth, C., Van Staden, J. An improved method of in vitro propagation ofDioscorea bulbifera . Plant Cell Tiss Organ Cult 1, 275–281 (1981). https://doi.org/10.1007/BF02318924
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DOI: https://doi.org/10.1007/BF02318924