Summary
Separation of paramagnetic substances under the field of magnetic gradient by using capillary tubing was attempted. Teflon tubing of 0.5 mm i.d. and 270 cm in length was placed on the outside of one pole of an electric magnet which had 9 K gauss at 30 V. The Samples were bovine serum albumin, egg albumin, and EDTA, and their complexes with Ni ion. The mobile phase was water at a flow rate of 20 μl/min. Samples without Ni ion had the same retention volume irrespective of the existence of magnetic gradient. Samples which formed complexes with Ni ion showed retardation under the field of magnetic gradient, although the difference of retention volumes obtained with and without the magnetic field was not large.
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Mori, S. Magnetic field-flow fractionation using capillary tubing. Chromatographia 21, 642–644 (1986). https://doi.org/10.1007/BF02311919
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DOI: https://doi.org/10.1007/BF02311919