Summary
A new chiral counter ion, N-benzoxycarbonyl-glycyl-L-proline (ZGP), added to the organic mobile phase (dichloromethane) has been used for separation of enantiomers of aminoalcohols with LiChrosorb DIOL as the solid phase. Separation factors of 1.2 to 1.4 for enantiomers of β-adrenergic blocking agents (e.g., alprenolol, metoprolol and propranolol) were obtained. Some structural requirements in the solutes and the counter ion essential for chiral resolution were observed. The retention was regulated by the concentration of counter ion or by the addition of triethylamine to the mobile phase. The chiral counter ion was utilized to determine the enantiomeric impurity of less than 0.1% in S-alprenolol and for the analysis of propranolol enantiomers in plasma samples.
Similar content being viewed by others
References
E. J. Ariëns, W. Soudijn, P. B. M. W. M. Timmermans (Editors), Stereochemistry and Biological Activity of Drugs, Blackwell Scientific Publications, Oxford, 1983.
W. F. Lindner, Chemical Derivatization in Analytical Chemistry, vol. 2, Ch. 4, Plenum Press, New York, 1982.
W. F. Lindner, C. Pettersson, Liquid Chromatography in Pharmaceutical Development, An Introduction Part 1. Aster Publishing Corp., Springfield, 1985.
B. Silber, S. Riegelman, J. Pharmacol. Exp. Ther.,215, 643 (1980).
J. Hermansson, Acta. Pharm. Suec.,19, 11 (1982).
I. W. Wainer, T. D. Doyle, K. H. Donn, J. R. Powell, J. Chromatogr.,306, 405 (1985).
C. Pettersson, G. Schill, J. Chromatogr.,204, 179 (1981).
L.-E. Arvidsson, Doctoral Thesis, University of Uppsala Sweden (1985).
C. Pettersson, J. Chromatogr.,316, 553 (1984).
C. E. Dalgliesh, J. Chem. Soc., 3940 (1952).
C. H. Lochmüller, R. W. Souter, J. Chromatogr.,113, 283 (1975).
V. A. Davankov, A. A. Kurganov, Chromatographia,17, 686 (1983).
M. M. Davis, The Chemistry of Nonaqueous Solvents (ed.J. L. Lagowski) Vol. III, Ch. 1, Academic Press, New York, 1970.
C. Pettersson, G. Schill, Chromatographia,16, 192 (1982).
C. Pettersson, K. No. J. Chromatogr.,282, 671 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pettersson, C., Josefsson, M. Chiral separation of aminoalcohols by ion-pair chromatography. Chromatographia 21, 321–326 (1986). https://doi.org/10.1007/BF02311603
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02311603