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The study of dielectrophoresis of cells using the optical technique of measuring

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Abstract

The present report gives the main results obtained in the studies of dielectrophoresis of cellular suspensions by the optical technique of registration. The description of this technique and its comparison with the earlier developed microscopic techniques of cell dielectrophoresis is given. It is shown that the frequency dependences of the orientation and pearl-chain-formation effect of cells in the electric field and the effect of their dielectrophoretic precipitation on the electrodes, called orientation and dielectrophoretic spectra, respectively, depend on the conductivity and pH of suspension as well as on the adsorption of substances on the cell surface. The conclusion is advanced that the method of cellular dielectrophoresis is a promising one for the analysis of electrical and morphological properties of cells and for the study of the influence of physical and chemical factors on the living cells.

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References

  1. Pohl, H.A. and Hawk, I. (1966)Science 152, 647.

    ADS  Google Scholar 

  2. Pohl, H.A. and Crane, J.S. (1971)Biophys. J. 11, 711.

    Google Scholar 

  3. Crane, J.S. and Pohl, H.A. (1972)J. Theor. Biol. 37, 15.

    Article  Google Scholar 

  4. Pohl, H.A. (1951)J. Appl. Phys. 22, 869.

    Article  Google Scholar 

  5. Pohl, H.A. (1968)J. Electrochem. Soc. 115, 155C.

    Google Scholar 

  6. Pohl, H.A. (1973)J. Biol. Phys. 1, 1.

    Article  Google Scholar 

  7. Pohl, H.A. (1973) inElectrostatics and its Applications (Moore, A.D., ed.) Chapter 14, p. 336.

  8. Pohl, H.A. and Crane, J.S. (1973) inElectrostatics and its Applications (Moore, A.D., ed.) Chapter 15, p. 363.

  9. Crane, J.S. and Pohl, H.A. (1968)J. Electrochem. Soc. 115, 584.

    Google Scholar 

  10. Chen, C.S.; Pohl, H.A.; Huebner, J.S.; and Bruner, L.W. (1971)J. Colloid Interface Sci. 37, 354.

    Article  Google Scholar 

  11. Chen, C.S. and Pohl, H.A. (1974)Ann. N.Y. Acad. Sciences 238, 176.

    Google Scholar 

  12. Petkanchin, I. and Stoylov, S.P. (1972) inSurface Forces in Thin Films and Disperse Systems, Moscow, Nauka, p. 132.

    Google Scholar 

  13. Miroshnikov, A.I. and Finakov, G.Z. (1974) inProblems of Technical Cybernetics of Biological Experiment, Pushchino, p. 94.

  14. Füredi, A.A. and Ohad, I. (1964)Biochim. Biophys. Acta 79, 1.

    Google Scholar 

  15. Degtyaryova, T.V.; Stepanov, G.V. and Kuznetsov, A.M. (1969)Information Bulletin of Coordinating Regional Council on Physiology and Biochemistry of Plants in Sibiria-Far East Zone. No. 4. Irkutsk, p. 30.

    Google Scholar 

  16. Teixera-Pinto, A.A.; Nejelski, L.L.; Cutler, J.Z.; and Heller, J.H. (1960)Exper. Cell Res. 20, 548.

    Google Scholar 

  17. Kasimenko, V.B. (1969)Doklady Akademii Nauk, BSSR 13, 730.

    Google Scholar 

  18. Tolstoy, N.A.; Spartakov, A.A.; Trusov, A.A. and Shekunova, S.A. (1966)Biophysica 11, 453.

    Google Scholar 

  19. Tolstoy, N.A.; Spartakov, A.A.; and Trusov, A.A. (1967) inStudies in the Field of Surface Forces, Moscow, Nauka, p. 56.

    Google Scholar 

  20. Tolstoy, N.A.; Spartakov, A.A.; Trusov, A.A.; and Vorontsov-Velyaminov, P.N. (1968) inStructure and Role of Water in Living Organism, Leningrad, 2.

  21. Jennings, B.R. and Morris, V.J. (1974)J. Colloid Interface Sci. 49, 89.

    Article  Google Scholar 

  22. Stoylov, S.P. (1971)Advances in Colloid Interface Science 3, 45.

    Google Scholar 

  23. Morris, V.J.; Rudd, P.J.; and Jennings, B.R. (1975)J. Colloid Interface Sci. 50, 379.

    Article  Google Scholar 

  24. Rudd, P.J.; Morris, V.J.; and Jennings, B.R. (1975)J. Phys. D.: Appl. Phys. 8, 170.

    Article  ADS  Google Scholar 

  25. Fomchenkov, V.M.; Miroshnikov, A.I.; Ivanov, A.Yu.; and Kuvshinnikova, V.A. (1975)Electronic Treatment of Materials 2, 60.

    Google Scholar 

  26. Sher, L.D. (1968)Nature 220, 695.

    Google Scholar 

  27. Saito, M. and Schwan, H.P. (1961) inBiological Effects of Microwave Radiation, v. 1, Plenum Press, p. 85.

  28. Gruzdev, A.D. (1965)Biofisika 10, 1091.

    Google Scholar 

  29. Schwarz, G.; Saito, M.; and Schwan, H.P. (1965)J. Chem. Phys. 43, 3562.

    Article  Google Scholar 

  30. Saito, M.; Schwan, H.P.; and Schwarz, G. (1966)Biophys. J. 6, 313.

    Google Scholar 

  31. Shilov, V.N. and Estrela-Lewis, V.R. (1972) inSurface Forces in Thin Films and Disperse Systems, Moscow, Nauka, p. 115.

    Google Scholar 

  32. Shilov, V.N.; Estrela-Lewis, V.R.; Stoylov, S.P.; and Dukhin, S.S. (1974)Kolloidny J. 36, 97.

    Google Scholar 

  33. Estrela-Lewis, V.R.; Shilov, V.N.; and Dukhin, S.S. (1974)Kolloidny J. 36, 328.

    Google Scholar 

  34. Tamm, I.E. (1976)The Principles of the Electricity Theory, Moscow, Nauka, p. 152.

    Google Scholar 

  35. Dukhin, S.S. and Shilov, V.N. (1972)Dielectrical phenomena and the Double Layer in Disperse Systems and Volyelectrolytes, Kiev, Naukova Dumka.

    Google Scholar 

  36. Dukhin, S.S. and Deryagin, B.V. (1976)Electrophoresis, Moscow, Nauka, p. 207.

    Google Scholar 

  37. Shilova, E.I. (1975) inMaterials of IIIrd All-Union Symposium “Effect of magnetic field on biological objects,” Kaliningrad, p. 27.

  38. Tolstoy, N.A. and Feofilov, P.P. (1949)Doklady Adad. Nauk USSR 66, 617.

    Google Scholar 

  39. Fomchenkov, V.M., Author's certificate No. 469748, Int. c1., c. 12, K 1/10.

  40. Miroshnikov, A.I.; Fomchenkov, V.M.; Gabujev, I.S.; and Chekanov, V.A. (1977)Separation of Cellular Suspensions, Moscow, Nauka, p. 115.

    Google Scholar 

  41. Lavrov, I.S.; Granovsky, M.G.; and Smirnov, O.V. (1972)Electronic Treatment of Materials 4, 47.

    Google Scholar 

  42. Fomchenkov, V.M.; Gavrilyuk, B.K.; and Miroshnikov, A.I. (in press)Biophizika.

  43. Fomchenkov, V.M. and Gavrilyuk, B.K. (1977)Studia biophysica 65, 35.

    Google Scholar 

  44. Bibik, E.E.; Efremov, I.F.; and Lavrov, I.S. (1964) inStudies on the Surface Forces, Moscow, Nauka, p. 265.

    Google Scholar 

  45. Forchenkov, V.M.; Brezgunov, V.N.; Boleznin, M.I.; and Smolayninov, V.V. (in press)Electronic Treatment of Materials.

  46. Fomchenkov, V.M.; Miroshnikov, A.I.; Ivanov, A.Yu.; and Kuvshinnikova, V.V. (1974) inBiology and Scientific and Technical Progress, Pushchino, p. 257.

  47. Schwan, H. (1957)Advances Biol. and Med. Phys. 5, 147.

    Google Scholar 

  48. Asami, K.; Hanai, T.; and Koizuni, N. (1976)J. Membrane Biol. 28, 169.

    Article  Google Scholar 

  49. Mason, B.D. and Towsnley, P.M. (1971)Can. J. Microbiol. 17, 879.

    Google Scholar 

  50. Gvozdyak, P.I. and Chekhovskaya, T.P. (1976)Mikrobiologiya 45, 901.

    Google Scholar 

  51. Gvozdyak, P.I.; Garbara, S.V.; et al. (1977)Mikrobiologiya 46, 118.

    Google Scholar 

  52. Gvozdyak, P.I., et al. (1974)Doklady Akad. Nauk, USSR 218, 970.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biological Physics, Academy of Sciences of the USSR, 142292, Pushchino, Moscow, USSR

    V. M. Fomchenkov & B. K. Gavrilyuk

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  1. V. M. Fomchenkov
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  2. B. K. Gavrilyuk
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Fomchenkov, V.M., Gavrilyuk, B.K. The study of dielectrophoresis of cells using the optical technique of measuring. J Biol Phys 6, 29–68 (1978). https://doi.org/10.1007/BF02311219

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