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Production and properties ofβ-glucosidase byNeurospora sitophila

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Abstract

Growth at 25°C and pH 5.50 favour the production ofβ-glucosidase. De-fatted oilseed flour and Tween 80 enhanced the production ofβ-glucosidase, Lactose, gentibiose, gentibiose-acetate, laminarabiose and xylobiose inducedβ-glucosidase activity. Precipitation of the culture filtrate with (NH4)2SO4 resulted in 26-fold purification with 67% recovery. The optimum pH and temperature for activity were 5.0 to 5.4 and 55°C respectively. The enzyme was stable at 40°C with half-life at 12 h at 50°C. TheK m andV max for the hydrolysis ofp-nitrophenyl-β-d-glucoside at 40°C H 5.0 are 0.28mm and 0.60 U/mg protein, respectively.

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Authors and Affiliations

  1. Department of Food Technology, University of Ibadan, Ibadan, Nigeria

    Gbekeloluwa B. Oguntimein

  2. Industrial Biotechnology Centre, Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada

    Murray Moo-Young

Authors
  1. Gbekeloluwa B. Oguntimein
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  2. Murray Moo-Young
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Oguntimein, G.B., Moo-Young, M. Production and properties ofβ-glucosidase byNeurospora sitophila . World J Microbiol Biotechnol 7, 4–11 (1991). https://doi.org/10.1007/BF02310911

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  • DOI: https://doi.org/10.1007/BF02310911

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