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High frequency recombination betweenloxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase

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Somatic Cell and Molecular Genetics

Abstract

An adenovirus vector (AdCre1) expressing Cre recombinase has been used to induce recombination betweenloxP sites in human chromosomes. G418 resistant cells with oneloxP site, generated by transfection with a plasmid containingloxP between the SV40 promoter and the G418 resistance (neo) gene, were infected with AdCre1 and transfected with a plasmid containingloxP adjacent to a promoterless hisD gene. This resulted in integration of hisD downstream of the SV40 promoter with gain of histidinol and loss of G418 resistance. Since AdCre1 is non-replicating and Cre expression transient, histidinol resistant cells containing the hisD gene flanked byloxP sites were stable. Reinfection of these cells with AdCre1 induced excision of hisD in over 90% of infected cells. This high efficiency of site-specific recombination suggests that AdCre1 may be exploited for temporal and tissue-specific regulation of gene expression and for chromosome engineering in vitro and in animals.

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Authors and Affiliations

  1. Department of Pathology, McMaster University, 1200 Main West, L8S 3Z5, Hamilton, Ontario, Canada

    Ping Wang, Frank L. Graham & Silvia Bacchetti

  2. Department of Biology, McMaster University, 1200 Main West, L8S 3Z5, Hamilton, Ontario, Canada

    Martina Anton & Frank L. Graham

Authors
  1. Ping Wang
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  2. Martina Anton
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  3. Frank L. Graham
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  4. Silvia Bacchetti
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Wang, P., Anton, M., Graham, F.L. et al. High frequency recombination betweenloxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase. Somat Cell Mol Genet 21, 429–441 (1995). https://doi.org/10.1007/BF02310209

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  • DOI: https://doi.org/10.1007/BF02310209

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