Abstract
The lipid content and fatty acid composition of a strain ofBacillus caldolyticus and of two facultative thermophiles (B. flavothermus and strain NZ-2) were analysed after growth at different temperatures. In all three strains the amount of membrane, as a fraction of total cellular dry mass, was found to increase with temperature, however, in varying degrees. Changes of lipid content and protein/lipid ratio inB. caldolyticus between 60°C and 100°C and in strain NZ-2 between 45°C and 70°C were minor; inB. f avothermus the alterations in the 50°C–70°C range were more pronounced. The same was found for changes observed in the phospholipid/total lipid and phospholipid/membrane ratios, and also in the amounts of individual phospholipids. The alterations of the fatty acid composition were most significant inB. caldolyticus, especially between 80°C and 95°C. In contrast, the main changes inB. flavothermus and NZ-2 were found to occur between 30°C and 50°C, and between 45°C and 60°C, respectively. Based on these data, strain NZ-2 could be characterized as the least andB. flavothermus as the most versatile of the three organisms.
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References
Bodman, H. andWelker, N. E. 1969. Isolation of spheroplast membranes and stability of spheroplasts ofBacillus stearothermophilus. -J. Bacteriol.97: 924–935.
De Bruyn, J. W., Van Keulen, H. A. andFerguson, J. H. A. 1968. Rapid method for the simultaneous determination of glucose and fructose using anthrone reagent. -J. Sci. Food Agric.19: 597–601.
Frank, G., Haberstich, H. U., Schaer, H. P., Tratshin, J. D. andZuber, H. 1976. Thermophific and mesophilic enzymes fromB. caldotenax andB. stearothermophilus: properties, relationships and formation. p. 375–389.In H. Zuber (ed.), Enzymes and Proteins from Thermophilic Microorganisms. -Birkäduser Verlag, Basel.
Hasegawa, Y., Kawada, N. andNosoh, Y. 1980. Change in chemical composition of membrane ofBacillus caldotenax after shifting the growth temperature. -Arch. Microbiol.126: 103–108.
Heinen, W., Klein, H. P. andVolkmann, C. M. 1970. Fatty acid composition ofThermus aquaticus at different growth temperatures. -Arch. Mikrobiol.72: 199–202.
Heinen, W. andLauwers, A. M. 1981. Growth of bacteria at 100°C and beyond. -Arch. Microbiol.129: 127–128.
Heinen, W. andLauwers, A. M. 1983. Changes in enzyme stability and fatty acid composition ofStreptomyces sp., a facultative thermophilic actinomycete. -Arch. Microbiol.134: 247–250.
Heinen, W., Lauwers, A. M. andMulders, J. W. M. 1982.Bacillus flavothermus, a newly isolated facultative thermophile. -Antonie van Leeuwenhoek48: 265–272.
Jackson, T. J., Ramaley, R. F. andMeinschein, W. G. 1973. Fatty acids of a non-pigmented, thermophilic bacterium similar toThermus aquaticus. -Arch. Mikrobiol.88: 127–133.
Johnston, N. C. andGoldfine, H. 1982. Effects of growth temperature on fatty acid and alk-l-enyl group compositions ofVeillonella parvula andMegasphaera elsdenii phospholipids. -J. Bacteriol.149: 567–575.
Kaneda, T. 1977. Fatty acids of the genusBacillus: an example of branched-chain preference. -Bacteriol. Rev.41: 391–418.
Kates, M. 1972. Techniques of lipidology. Chapt. 5. Techniques for separation of lipid mixtures. p. 393–469.In T. S. Work and E. Work (eds), Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 3. -North-Holland Publ. Co., Amsterdam.
Lauwers, A. M. andHeinen, W. 1983. Thermal properties of enzymes fromBacillus flavothermus, grown between 34 and 70°C. -Antonie van Leeuwenhoek49: 191–201.
Lauwers, A. M., Heinen, W. andMulders, J. W. M. 1981. Properties of enzymes from bacteria grown in the 70–100°C range. -Arch. Microbiol.130: 159–164.
Rouser, G., Fleischer, S. andYamamoto, A. 1970. Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. -Lipids5: 494–496.
Salton, M. R. J. andFreer, J. H. 1965. Composition of the membranes isolated from several gram-positive bacteria. -Biochim. Biophys. Acta107: 531–538.
Sinensky, M. 1974. Homeoviscous adaptation — a homeostatic process that regulates the viscosity of membrane lipids inEscherichia coli. -Proc. Natl Acad. Sci. USA71: 522–525.
Souza, K. A., Kostiw, L. L. andTyson, B. J. 1974. Alterations in normal fatty acid composition in a temperature-sensitive mutant of a thermophilecBacillus. -Arch. Microbiol.97: 89–102.
Weerkamp, A. andHeinen, W. 1972. Effect of temperature on the fatty acid composition of the extreme thermophiles,Bacillus caldolyticus andBacillus caldotenax. -J. Bacteriol.109: 443–446.
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Aerts, J.M.F.G., Lauwers, A.M. & Heinen, W. Temperature-dependent lipid content and fatty acid composition of three thermophilic bacteria. Antonie van Leeuwenhoek 51, 155–165 (1985). https://doi.org/10.1007/BF02310009
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DOI: https://doi.org/10.1007/BF02310009