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Reversed phase, high-performance liquid chromatography: Effect of the structure of the chemically bonded hydrocarbon ligand on retention and selectivity

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Summary

Chemically bonded stationary phases (CBSP) were prepared by reaction of di- and trialkylchloro- and methoxy-silanes, R1R2SiCl2 and R1R2Si(OMe)2 with varying chain length of the ligands R1 and R2 with Lichrosorb Si 100, 10 μm, Merck. The influence of the structure of the CBSPs on the retention and selectivity in reversed phase chromatography (RPC) was investigated and compared with results obtained with monoalkyl-CPSPs. Plots of log k′ versus the percentage carbon yield straight lines. The slopes and intercepts are dependent on the structure of the ligands and the structure of the solute. An attempt was made to explain how structure of the CBSP influences the selectivity in RPC.

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References

  1. G. A. Howard andA. J. P. Martin, Biochem. J.56, 539 (1950).

    Google Scholar 

  2. I. Halász andI. Sebestian, Angew. Chem.8, 453 (1969).

    Google Scholar 

  3. V. Rehák andE. Smolková, Chromatographia9, 219 (1976).

    Google Scholar 

  4. H. Colin andG. Guiochon, J. Chromatogr.141, 289 (1977).

    Article  CAS  Google Scholar 

  5. R. E. Majors andM. J. Hopper, J. Chromatogr. Sci.12, 767 (1974).

    CAS  Google Scholar 

  6. K. K. Unger, N. Becker andP. Roumeliotis, J. Chromatogr.125, 115 (1976).

    Article  CAS  Google Scholar 

  7. K. Karch, I. Sebestian, I. Halász andH. Engelhardt, J. Chromatogr.122, 171 (1976).

    CAS  Google Scholar 

  8. H. Hemetsberger, W. Maasfeld andH. Ricken, Chromatographia9, 303 (1976).

    Article  CAS  Google Scholar 

  9. H. Hemetsberger, M. Kellermann andH. Ricken, Chromatographia10, 726 (1977).

    Article  CAS  Google Scholar 

  10. D. C. Locke, J. T. Schmermund andB. Banner, Anal. Chem.44, 90 (1972).

    Article  Google Scholar 

  11. D. C. Locke, J. Chromatogr. Sci.11, 120 (1973).

    CAS  Google Scholar 

  12. St. R. Bakalayar, R. McIlwrick andE. Roggendorf, J. Chromatogr.142, 353 (1977).

    Google Scholar 

  13. C. Horvath, W. Melander andI. Molnar, J. Chromatogr.125, 129 (1976).

    CAS  Google Scholar 

  14. B. L. Karger, J. R. Gant, A. Hartkopf andP. H. Weiner, J. Chromatogr.128, 65 (1976).

    Article  CAS  Google Scholar 

  15. O. Sinanoĝlu, inPullman (ed.), Molecular Associations in Biology, Academic Press, New York, pp. 427–445.

  16. O. Sinanoĝlu andS. Abdulnur, Fed. Proc.24 (2), 12 (1965).

    Google Scholar 

  17. R. M. Cassidy, D. S. LeGay andR. W. Frei, Anal. Chem.46, 340 (1974).

    Article  CAS  Google Scholar 

  18. I. Halász andI. Sebestian, Chromatographia7, 371 (1974).

    Article  Google Scholar 

  19. I. Halász, inJ. J. Kirkland, “Modern Practice of Liquid Chromatography”, Wiley-Interscience, New York, 1971.

    Google Scholar 

  20. B. L. Karger, inJ. J. Kirkland, “Modern Practice of Liquid Chromatography”, Wiley-Interscience, New York, 1971.

    Google Scholar 

  21. K. Unger andE. Gallei, Kolloid and Polymere237, 358 (1970).

    CAS  Google Scholar 

  22. K. Unger, Angew. Chem., Int. Ed.11, 267 (1972).

    CAS  Google Scholar 

  23. K. Unger, K. Berg, E. Gallei andG. Erdee, Fortschr. Kolloide Polym.55, 34 (1971).

    CAS  Google Scholar 

  24. K. Karch, I. Sebestian andI. Halász, J. Chromatogr.122, 3 (1976).

    CAS  Google Scholar 

  25. J. H. Knox andA. Pryde, J. Chromatogr.112, 171 (1975).

    Article  CAS  Google Scholar 

  26. L. B. Kier andL. H. Hall, Molecular Connectivity in Chemistry and Drug Research, Academic Press, New York, San Francisco, London, 1976.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Organic Chemistry II, Ruhr-University Bochum, D-4630, Bochum, Federal Republic of Germany

    H. Hemetsberger, P. Behrensmeyer, J. Henning & H. Ricken

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  1. H. Hemetsberger
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  2. P. Behrensmeyer
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  3. J. Henning
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  4. H. Ricken
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Hemetsberger, H., Behrensmeyer, P., Henning, J. et al. Reversed phase, high-performance liquid chromatography: Effect of the structure of the chemically bonded hydrocarbon ligand on retention and selectivity. Chromatographia 12, 71–76 (1979). https://doi.org/10.1007/BF02302929

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  • DOI: https://doi.org/10.1007/BF02302929

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