Summary
The properties of chloroform as a liquid stationary phase in reversed-phase liquid chromatography have been investigated. Stable systems and complete pore filling of the support were obtained. Uncharged acids were slightly adsorbed by the support. Amines in the cationic form showed tailing, which can be attributed to adsorption on the support material. The influence of several reversed-phase supports on retention and peak symmetry has been studied. Little difference was found for uncharged acids, but retention and peak shape of the cations was dependent on the specific support. Also on the poly(styrene-divinylbenzene) copolymer PRP-1 cations gave tailing peaks. The results indicate that the surface of reversed-phase supports is heterogeneous. From this and other studies it can be concluded that only certain reversed-phase silicas, such as μ-Bondapak, are suitable supports for cationic solutes. In liquid-liquid chromatography polar solutes only obey a liquid-liquid distribution model if the liquid stationary phase molecules contains a hydrophobic group, as well as a polar function.
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Stuurman, H.W., Pettersson, C. & Heldin, E. Adsorption of polar solutes in reversed-phase liquid-liquid chromatography. Chromatographia 25, 685–690 (1988). https://doi.org/10.1007/BF02290471
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DOI: https://doi.org/10.1007/BF02290471