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Separation and characterisation of hop bitter acids by HPLC-1H NMR coupling

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Summary

A supercritical carbon dioxide hop extract has been analysed using on-line coupling of high performance liquid chromatography (HPLC) with1H nuclear magnetic resonance (NMR) spectroscopy. For the first time high quality NMR spectra of the six major hop bitter acids were recorded by employing the stopped-flow detection mode. The resolution of the spectra obtained is similar to that of conventionally recorded spectra. All data are in agreement with the known structures.

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References

  1. M. Verzele, D. De Keukeleire, eds, “Developments in Food Sciences, Vol. 27: Chemistry and Analysis of Hop and Beer Bitter Acids”, Elsevier, Amsterdam, 1991, p. 9.

    Google Scholar 

  2. A. C. J. Hermans-Lokkerbol, R. Verpoorte, J. Chromatogr. A664, 45 (1994).

    Google Scholar 

  3. U. Rust, SKW Trostberg AG, Trostberg, 1995, personal communication.

  4. R. Vandenbossche, Ph. D. Thesis, Lab. Org. Chem., University Gent, 1973.

  5. K. Albert, J. Chromatogr.703, 123 (1995).

    Google Scholar 

  6. K. Albert, E. Bayer, “Analytical Methods and Instrumentation (AMI)”, in press.

  7. G. A. Howard, A. R. Tatchell, J. Chem. Soc. 2400 (1954).

  8. G. A. Howard, A. R. Tatchell, Chem. Ind. (London) 514 (1954).

  9. G. A. Howard, A. R. Tatchell, Chem. Ind. (London) 992, (1954).

  10. W. Riedl, J. Nickl, Ber.89, 1863 (1956).

    Google Scholar 

  11. W. Riedl, J. Nickl, R. H. Risse, R. Mitteldorf, Ber.59, 1849 (1956).

    Google Scholar 

  12. G. Alderton, G. F. Bailey, J. C. Lewis, F. Stitt, Anal. Chem.26, 983 (1954).

    Google Scholar 

  13. H. Claus, J. Van Dyck, M. Verzele, J. Inst. Brew.84, 218 (1978).

    Google Scholar 

  14. M. Verzele, N. van de Velde, J. Chromatogr.387, 473 (1987).

    Google Scholar 

  15. A. Höltzel, “Analytik von Hopfenbitterstoffen”, Diploma Thesis, Universität Tübingen, Germany, 1995.

    Google Scholar 

  16. WIN-NMR, Bruker Franzen Analytik GmbH, Bremen, Germany.

  17. EBC-Analytica addendum, Monatsschr. Brauwiss.3, 129 (1992).

    Google Scholar 

  18. M. Verzele, D. De Keukeleire, eds, “Developments in Food Science, Vol. 27: Chemistry and Analysis of Hop and Beer Bitter Acids”, Elsevier, Amsterdam, 1991, pp. 21, 29, 30, 206.

    Google Scholar 

  19. B. E. Connett, J. A. Elvidge, J. Chem. Soc. (C) 340 (1969).

  20. M. Collins, D. R. J. Laws, J. D. McGuinness, J. A. Elvidge, J. Chem. Soc. (C) 3814 (1971).

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Authors and Affiliations

  1. Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 18, 72076, Tübingen, Germany

    A. Höltzel, G. Schlotterbeck, K. Albert & E. Bayer

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  1. A. Höltzel
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  2. G. Schlotterbeck
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  3. K. Albert
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  4. E. Bayer
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Höltzel, A., Schlotterbeck, G., Albert, K. et al. Separation and characterisation of hop bitter acids by HPLC-1H NMR coupling. Chromatographia 42, 499–505 (1996). https://doi.org/10.1007/BF02290283

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  • DOI: https://doi.org/10.1007/BF02290283

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