Summary
The diversity of antibodies in patients with scleroderma, mixed connective tissue disease or “primary” Raynaud's phenomenon could be used as a laboratory aid in the clinical diagnosis. In serum samples of 75 patients we screened for antinuclear antibodies (HEp 2 cells), anti DNA, soluble nucleoprotein and extractable nuclear antigens (Sm, rRNP, Ul-nRNP, SSA/Ro, SSB/La and Scl-70). Distinctive antinuclear antibodies pattern was identified in each group of patients. This immunologic profile is valuable for clinical diagnosis and the preferential association of certain autoantibodies with some diseases and not with others, suggest an antigen-driven stimulus for its production.
Similar content being viewed by others
References
Rodnan, G.P. Progressive systemic sclerosis (Scleroderma) Ch. 53 in Arthritis and Allied Conditions, 9th ed., Editor: McCarty, D.J. Philadelphia, Lea and Febiger, 1979, 762.
Sharp, G.C., Irvin, W.S., Tan, E.M., Gould, R.G., Holman, H.R. Mixed connective tissue disease-an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA) Am J Med 52, 1972, 148–159.
Nimelstein, S.H., Brody, S., McShane, D., Holman, H.R.: Mixed connective tissue disease: A subsequent evaluation of the original 25 patients. Medicine 1980, 59, 239–248.
Gerbracht, D.D., Steen, V.D., Ziegler, G.L., Medsger, T.A., Rodnan, G.P. Evolution of primary Raynaud's phenomenon (Raynaud's disease) to connective tissue disease. Arthritis Rheum 1985, 28, 87–92.
Tan, E.M., Rodnan, G.P., Garcia, I., Moroi, Y., Fritzler, M.J., Peebles, C. Diversity of antinuclear antibodies in progressive systemic sclerosis. Arthritis Rheum 1980, 23, 617–625.
Tan, E.M. Antinuclear antibodies in scleroderma. Int J Dermatol 1981, 20, 569–574.
Kleinsmith, A.M., Heinzerling, R.H., Bumham, T.K. Antinuclear antibodies as immunologic markers for a benign subset and different clinical characteristics of scleroderma. Arch Dermatol 1982, 118, 882–885.
Chen, Z.Y., Silver, R.M., Ainsworth, S.K., Dobson, R.L., Rust, P., Maricq, H.R. Association between fluorescent antinuclear antibodies, capillary patterns, and clinical features in scleroderma spectrum disorders. Am J Med 1984, 77, 812–822.
Masi, A.T. Preliminary criteria for the classification of systemic sclerosis (scleroderma) Arthritis Rheum 1980, 23, 581–590.
Winterbauer, R.H. Multiple telangiectasia, Raynaud's phenomenon, sclerodactily, and subcutaneous calcinosis: a syndrome mimicking hereditary telangiectasia. Johns Hopkins Med J, 1964, 114, 361–383.
Carr, R.D., Heisel, E.G., Stevenson, T.D. CREST Syndrome: a benign variant of scleroderma. Arch Dermatol 1965, 92, 519–524.
LeRoy, E.C. A prospective study of Raynaud's phenomenon and early connective tissue disease. Am J Med 1982, 72, 883–888.
Alarçón Segovia, D., Villarreal, M. Classification and diagnostic criteria for mixed connective tissue disease. In: Mixed Connective Tissue Disease and Anti-Nuclear Antibodies. Editors: Reiji-Kasukawa Gordon C. Sharp. Ed. Experta Medica 1987, 719, 33–40.
Inami, Y.H., Nakamura, R.N. Hemaglutination test for detection of antibodies to deoxyribonucleic acid and nucleoprotein. In: Manual of Clinical Rheumatology, 2nd ed., chap. 115. Editor: Rose, N.R., Friedman, H., Washington, D.C., Am. Soc. Microbiology, 1980, 862.
Notman, D.D., Kurata, N., Tan, E.M. Profiles of antinuclear antibodies in systemic rheumatic diseases. Ann Intern Med 1975, 83, 464–469.
Clark, G., Reichlin, M., Tomasi, T.B. Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythematosus. J Immunol 1968, 102: 117–122.
Littlefield, J.W., Keller, E.B., Gross, J., Zamecnik, P.C. Studies of cytoplasmic ribonucleoprotein particles from the liver of the rat. J Biol Chem 1955, 217, 111–123.
Bernstein, R.M., Steigerwald, J.C., Tan, E.M. Association of antinuclear and antinucleolar antibodies in progressive systemic sclerosis. Clin Exp Immunol 1982, 48, 43–51.
Stee, V.D., Ziegler, G.L., Rodnan, G.P., Medsger, T.A. Clinical and laboratory associations of anticentromere antibody in patients with progresive systemic sclerosis. Arthritis Rheum 1984, 27, 125–131.
Fritzler, M.J., Kinsella, T.D., Garbutt, E. The CREST syndrome: a distinct serologic entity with anticentromere antibodies. Am J Med 1980, 69, 520–526.
Moroi, Y., Peebles, C., Fritzler, M.J., Steigerwald, J., Tan, E.M. Autoantibody to centromero (kinetochore) in scleroderma sera. Proc Natl Acad Sci USA, 1980, 77, 1627–1631.
Tuffanelli, D.L., McKeon, F., Kleinsmith, A.M., Burham, T.K., Kirschner, M. Anticentromere and anticentriole antibodies in the scleroderma spectrum. Arch Dermatol 1983, 119, 560–566.
McCarty, G.A., Rice, J.R., Bembe, M.L., Barada, F. Anticentromere antibody. Clinical correlations and association with favorable prognosis in patients with scleroderma variants. Arthritis Rheum 1983, 26, 1–7.
Nishikai, M., Okano, Y., Yamashita, H., Watanabe, M. Characterization of centromere (kinetochore) antigen reactive with sera of patients with a scleroderma variant (CREST Syndrome) Ann Rheum Dis 1984, 43, 819–824.
Powell, F.C., Winkelmann, R.K., Venencie Le Marchand, F., Spurkeck, J.L., Schroeter, A.L. The anticentromere antibody: disease specificity and clinical significance. Mayo Clin Proc 1984, 59, 700–706.
Lerner, M.R., Steitz, J.A. Antibodies to small nuclear. RNA's complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 1979, 76, 5495–5499.
Rao, K.V., Berkseth, R.O., Crosson, J.T., Ray, L., Shapiro, F.L. Immune complex nephritis in mixed connective tissue disease. Ann Intern Med 1976, 84, 174–176.
Fuller, T.J., Richman, A.V., Auerbach, D., Alexander, R.W., Lottenberg, R., Longley, S. Immune-complex glomerulonephritis in a patient with mixed connective tissue disease. Am J Med 1977, 62, 761–764.
Bennett, R.M., Spargo, B.H. Immune complex nephropathy in mixed connective tissue disease. Am J Med 1977, 63, 534–541.
Singsen, B.H., Swanson, V.L., Bernstein, B.H., Heuser, E.T., Hanson, V., Landing, B.H.: A histologic evaluation of mixed connective tissue disease in childhood. Am J Med 1980, 68, 710–717.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cruz, M., Mejia, G., Lavalle, C. et al. Antinuclear antibodies in scleroderma, mixed connective tissue disease and “primary” Raynaud's phenomenon. Clin Rheumatol 7, 80–86 (1988). https://doi.org/10.1007/BF02284061
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02284061