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Sensitive pulsed amperometric detection of free and conjugated bile acids in combination with gradient reversed-phase HPLC

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Summary

A rapid and sensitive method has been developed for the simultaneous determination of free bile acids and its glyco-and tauro-conjugates by high-performance liquid chromatography (HPLC). These components were separated on a resin-based reversed-phase column, with a gradient of acetonitrile in dilute sodium hydroxide solution. The detection was performed at a gold electrode with a sequence of three pulses, which enabled the selective detection of hydroxyl-containing compounds, without fouling of the electrode. Detection limits ranged from 30 to 60 pmol oncolumn. Linear calibration graphs were obtained for bile acid solutions of 0.6 to 14 μmol/l. The method was applied to the determination of bile acids in culture media, bile, and ileal fluid.

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Authors and Affiliations

  1. Netherlands Institute for Dairy Research, P.O. Box 20, 6710 BA, Ede, The Netherlands

    R. Dekker, R. van der Meer & C. Olieman

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  1. R. Dekker
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  2. R. van der Meer
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  3. C. Olieman
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Dekker, R., van der Meer, R. & Olieman, C. Sensitive pulsed amperometric detection of free and conjugated bile acids in combination with gradient reversed-phase HPLC. Chromatographia 31, 549–553 (1991). https://doi.org/10.1007/BF02279474

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  • DOI: https://doi.org/10.1007/BF02279474

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