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Gibberellin biosynthetic pathway and the physiologically active gibberellin in the shoot ofCucumis sativus L.

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Abstract

[2H2]Gibberellin A24 (GA24) and [2H4]-GA9 were applied to the apices of normal-type cucumber (Cucumis sativus L. cv. Yomaki) seedlings treated with uniconazole, an inhibitor of GA biosynthesis. The metabolites from these feeds were identified by full-scan gas chromatography-mass spectrometry (GC-MS) to confirm the conversions of [2H2]GA24 to [2H2]GA9 and of [2H4]GA9 to [2H4]GA4. The results show that GA4 is biosynthesized from GA24 via GA9. In a cucumber hypocotyl elongation bioassay using cv. Yomaki, prohexadione (DOCHC), an inhibitor of 2-oxoglutaratedependent dioxygenase, inhibited the hypocotyl elongation caused by application of GA9, while DOCHC enhanced the elongation caused by application of GA4. These results indicate that GA4 is a physiologically active GA and that the activity of GA9 is due to its conversion to GA4 in cucumber shoots.

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Author notes

  1. Nobutaka Takahashi

    Present address: Department of Agricultural Chemistry, Utsunomiya University, 350 Mine-machi, 321, Utsunomiya, Japan

  2. Hideharu Seto

    Present address: The Institute of Physical and Chemical Research, 351-01, Wako-shi, Saitama, Japan

Authors and Affiliations

  1. Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan

    Masayoshi Nakayama, Hisakazu Yamane, Noboru Murofushi & Nobutaka Takahashi

  2. Research School of Chemistry, The Australian National University, GPO Box 4, 2601, Canberra, ACT, Australia

    Lewis N. Mander & Hideharu Seto

Authors
  1. Masayoshi Nakayama
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  2. Hisakazu Yamane
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  3. Noboru Murofushi
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  4. Nobutaka Takahashi
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  5. Lewis N. Mander
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  6. Hideharu Seto
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Nakayama, M., Yamane, H., Murofushi, N. et al. Gibberellin biosynthetic pathway and the physiologically active gibberellin in the shoot ofCucumis sativus L.. J Plant Growth Regul 10, 115–119 (1991). https://doi.org/10.1007/BF02279322

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  • DOI: https://doi.org/10.1007/BF02279322

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