Skip to main content
SpringerLink
Log in

Conversion of 1′-deoxy-2H-ABA to2H-ABA in cell-free extracts fromCercospora rosicola

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Abscisic acid (ABA) biosynthesis in the fungusCercospora rosicola has been studied previously by feeding potential intermediates to growing cultures and examining the products. The present work describes the isolation and partial characterization of an enzyme system which catalyzes the last step in this pathway, the conversion of 1′-deoxy-2H-ABA to2H-ABA. Enzyme extracts were prepared from cold-pressed mycelia of actively growingC. rosicola in tricine buffer containing sucrose and β-mercaptoethanol. Low-speed supernatants and resuspended microsomal preparations were active in converting 1′-deoxy-2H-ABA to2H-ABA. Optimum conditions for the reactions were established at 10 μM substrate, 300 μl enzyme extract in a total volume of 1 ml. Reaction products were chromatographed by reverse-phase high-performance liquid chromatography (HPLC). The presumptive ABA fractions were collected and the2H-ABA was measured by combined gas chromatography-mass spectrometry (GC-MS) with an2H-ABA standard. Most of the enzyme activity was found in the microsomal fraction. Typical reaction rates were on the order of 1.5 pmol2H-ABA formed/min/mg protein. The reaction required NADPH and was enhanced by FAD. Triarimol, a substituted pyrimidine, which inhibits ABA synthesis inC. rosicola, had no effect on the conversion of 1′-deoxy-2H-ABA to2H-ABA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Al-Nimri LF, Coolbaugh RC (1990) Inhibition of absisic acid biosynthesis inCercospora rosicola by triarimol. J. Plant Growth Regul 9:221–225

    Article  CAS  Google Scholar 

  • Assante G, Merlini L, Naisini G (1977) (+)-Abscisic acid, a metabolite of the fungusCercospora rosicola. Experientia 33:1556–1557

    Article  CAS  Google Scholar 

  • Bennett RD, Norman SM, Maier VP (1984) Biosynthesis of abscisic acid from farnesol derivatives inCercospora rosicola. Phytochemistry 23:1913–1915

    Article  CAS  Google Scholar 

  • Coolbaugh RC, Heil DR, West CA (1982) Comparative effects of substituted pyrimidines on growth and gibberellin biosynthesis inGibberella fujikuroi. Plant Physiol 69:712–716

    PubMed  CAS  Google Scholar 

  • Gage DA, Fong F, Zeevaart JAD (1989) Abscisic acid biosynthesis in isolated embryos ofZea mays L. Plant Physiol 89:1039–1041

    PubMed  CAS  Google Scholar 

  • Horgan R, Neill SJ, Walton DC, Griffin D (1983) Biosynthesis of abscisic acid. Biochem Soc Trans 11:553–557

    PubMed  CAS  Google Scholar 

  • Li Y, Walton DC (1987) Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves. Plant Physiol 85:910–915

    Article  PubMed  CAS  Google Scholar 

  • Li Y, Walton DC (1990) Violaxanthin is an abscisic acid precursor in water-stressed dark-grown bean leaves. Plant Physiol 92:551–559

    PubMed  CAS  Google Scholar 

  • Milborrow BV (1974) Biosynthesis of abscisic acid by a cell-free system. Phytochemistry 13:131–136

    Article  CAS  Google Scholar 

  • Neill SJ, Horgan R (1983) Incorporation of α-ionylideneethanol and α-ionylideneacetic acid into abscisic acid byCercospora rosicola. Phytochemistry 22:2469–2472

    Article  CAS  Google Scholar 

  • Neill SJ, Horgan R, Lee TS, Walton DC (1981) 3-Methyl-5-(4′-oxo-2′,6′,6′-trimethylcyclohex-2′-en-1′-yl)-2,4 pentadienoic acid, a putative precursor of abscisic acid fromCercospora rosicola. FEBS Lett 128:30–32

    Article  CAS  Google Scholar 

  • Neill SJ, Horgan R, Walton DC, Lee TS (1982a) The biosynthesis of abscisic acid inCercospora rosicola. Phytochemistry 21:61–65

    Article  CAS  Google Scholar 

  • Neill SJ, Horgan R, Walton DC, Griffin D (1982b) Biosynthesis of abscisic acid. In: Waring PE (ed) Plant growth substances 1982. Academic Press, London, pp 315–323

    Google Scholar 

  • Neill SJ, Horgan R, Walton DC (1984) Biosynthesis of abscisic acid. In: Biosynthesis and metabolism of plant hormones. (Seminar series/Society for Experimental Biology; 23): 43–70. Alan Crozier and John R. Hillman, eds., Cambridge University Press, New York

    Google Scholar 

  • Neill SJ, Horgan R, Walton DC, Mercer CAM (1987) The metabolism of α-ionylidene compounds byCercospora rosicold. Phytochemistry 26:2515–2519

    Article  CAS  Google Scholar 

  • Norman SM, Maier VP, Echols LC (1981) Development of a defined medium for growth ofCercospora rosicola Passerini. Appl Environ Microbiol 41:334–336

    PubMed  CAS  Google Scholar 

  • Norman SM, Poling SM, Maier VP, Nelson MD (1985) Ionylideneacetic acids and abscisic acid biosynthesis byCercospora rosicola. Agric Biol Chem 49:2317–2324

    CAS  Google Scholar 

  • Sindhu RK, Walton EC (1987) Conversion of xanthoxin to abscisic acid by cell-free preparations from bean leaves. Plant Physiol 85:916–921

    PubMed  CAS  Google Scholar 

  • Sindhu RK, Griffin DH, Walton DC (1990) Abscisic aldehyde is an intermediate in the enzymatic conversion of xanthoxin to abscisic acid inPhaseolus vulgaris L. leaves. Plant Physiol 93:689–694

    PubMed  CAS  Google Scholar 

  • Zeevaart JAD, Heath TG, Gage DA (1989) Evidence for a universal pathway of abscisic acid biosynthesis in higher plants from18O incorporation patterns. Plant Physiol 91:1594–1601

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Laila F. Al-Nimri

    Present address: Department of Biology, Yarmouk University, Irbid, Jordan

Authors and Affiliations

  1. Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, Indiana, USA

    Laila F. Al-Nimri & Ronald C. Coolbaugh

Authors
  1. Laila F. Al-Nimri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ronald C. Coolbaugh
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Journal Paper Number 12555 of the Purdue University Agricultural Experiment Station.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al-Nimri, L.F., Coolbaugh, R.C. Conversion of 1′-deoxy-2H-ABA to2H-ABA in cell-free extracts fromCercospora rosicola . J Plant Growth Regul 10, 63–66 (1991). https://doi.org/10.1007/BF02279313

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02279313

Keywords

Search

Navigation