Abstract
Contradictory results have been reported for the effects of triazoles on abscisic acid (ABA) levels in plants. Paclobutrazol reduced the height, fresh weight, and ABA levels of wheat seedlings. The magnitude of the inhibitory effect of paclobutrazol on ABA levels was dependent on the length of time after application. ABA levels in plants as determined by gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) were reduced by 50–60% 2 days after soil application. A further 15% reduction occurred by the seventh day after treatment. ABA level analyses began at the time of treatment with 4-day-old seedlings and continued until 21 days after treatment when only a 20% reduction was detected. These determinations using GC-SIM-MS should increase the understanding of triazole effects on ABA levels.
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References
Asare-Boamah NK, Hofstra G, Fletcher RA, Dumbroff EB (1986) Triadimefon protects bean plants from water stress through its effect on abscisic acid. Plant Cell Physiol 27:383–390
Cohen JD, Bausher MG, Bialek K, Buta JG, Gocal GFW, Janzen LM, Pharis RP, Reed AN, Slovin JP (1987) Comparison of a commercial ELISA assay for indole-3-acetic acid at several stages of purification and analysis by gas chromatography-selected ion monitoring-mass spectrometry using a13C6-labeled internal standard. Plant Physiol 84:982–986
Dalziel J, Laurence DK (1984) Biochemical and biological effects of kaurene oxidase inhibitors, such as paclobutrazol. Br Plant Growth Regul Group 11:43–57
Grossman K, Kwiatkowski J, Siebecker H, Jung J (1987) Regulation of plant morphology by growth retardants. Plant Physiol 84:1018–1021
Hedden P, Graebe JE (1985) Inhibition of gibberellin biosynthesis by paclobutrazol in cell-free homogenates ofCucurbita maxima endosperm andMalus pumila embryos. J Plant Growth Regul 4:111–122
Hofstra G, Kreig LC, Fletcher RA (1989) Uniconazole reduces ethylene and 1-aminocyclopropane-1-carboxylic acid and increases spermine levels in mung bean seedlings. J Plant Growth Regul 8:45–51
Izumi K, Nakagawa S, Kobayashi M, Oshio H, Sakurai A, Takahashi N (1988) Levels of IAA cytokinins, ABA and ethylene in rice plants as affected by a gibberellin biosynthesis inhibitor uniconazole-p. Plant Cell Physiol 29:97–104
Jung J, Luib M, Sauer H, Zeh B, Rademacher W (1987) Growth regulation in crop plants with new types of triazole compounds. J Agron Crop Sci 158:324–332
Lurssen K (1987) Physiological effects of traizole PGRs in relation to their biochemical mode of action. Pesticide Sci 21:310–311
Norman SM, Bennett RD, Poling SM, Maeir VP, Nelson MD (1986) Paclobutrazol inhibits abscisic acid biosynthesis ofCercospora rosicola. Plant Physiol 80:122–125
Rajasekaran K, Hein MH, Vasil IK (1987) Endogenous abscisic acid and indole-3-acetic acid and somatic embryogenesis in cultured leaf explants ofPennisetum purpureum Schum. Plant Physiol 84:47–51
Rivier L, Milon H, Pilet PE (1977) Gas chromatography-mass spectrometric determinations of abscisic acid levels in the cap and apex of maize roots. Planta 134:23–27
Wang SY, Sun T, Ji ZL, Faust M (1987) Effect of paclobutrazol on water-stress induced abscisic acid in apple seedling leaves. Plant Physiol 84:1051–1054
Zeevaart JAD, Heath TG, Gage DA (1989) Evidence for a universal pathway of abscisic acid biosynthesis in higher plants from18O incorporation patterns. Plant Physiol 91:1594–1601
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Buta, J.G., Spaulding, D.W. Effect of paclobutrazol on abscisic acid levels in wheat seedlings. J Plant Growth Regul 10, 59–61 (1991). https://doi.org/10.1007/BF02279312
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DOI: https://doi.org/10.1007/BF02279312