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Culture of epithelial cells from bovine ruminal mucosa

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Abstract

A method is reported for the primary in vitro culture of epithelial cells derived from bovine ruminal mucosa. That the cultures of ruminal epithelial cells consisted exclusively of stratum spinosum, stratum basale and stratum granulosum was confirmed by immunoperoxidase and immunofluorescence staining using carbonic anhydrase isoenzyme as a marker.

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References

  • Armstrong, J.McD., Myers, D.V., Verpoorte, J.A. and Edsall, J.T., 1966. Purification and properties of human erythrocyte carbonic anhydrase. J. Biol. Chem., 241: 5137–5149.

    Google Scholar 

  • Burns, I., Hambridge, M. and Taylor, C.R., 1974. Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates. J. Clin. Path., 27: 548–557.

    Google Scholar 

  • Carter, M.J., 1971. The carbonic anhydrase in the rumen epithelial tissue of the ox. Biochim. Biophys. Acta, 235: 222–236.

    Google Scholar 

  • Gay, C.V., Faleski, E.J., Schraer, H. and Schraer, R., 1974. Localisation of carbonic anhydrase in avian gastric mucosa, shell gland and bone by immunohistochemistry. J. Histochem. Cytochem., 22: 819–825.

    Google Scholar 

  • Kutas, F., Gálfi, P. and Neogrády, S., 1980. Study on the metabolism of epithelial cells isolated from rumen mucosa of cattle. XXVIII. International Congress of Physiological Sciences, Budapest, Hungary.

  • Lindskog, S., 1960. Purification and properties of bovine erythrocyte carbonic anhydrase. Biochim. Biophys. Acta, 39: 218–226.

    Google Scholar 

  • Stevens, C.E., 1970. Fatty acid transport through the rumen epithelium. In: A.T. Phillipson (Editor), Physiology of Digestion and Metabolism in the Ruminant. Newcastle, Oriel, 101 pp.

  • Streefkerk, J.G., 1972. Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. J. Histochem. Cytochem., 20: 829–831.

    Google Scholar 

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Gálfi, P., Neogrády, S. & Kutas, F. Culture of epithelial cells from bovine ruminal mucosa. Vet Res Commun 4, 295–300 (1980). https://doi.org/10.1007/BF02278507

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